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dc.contributor.authorCouso, Inmaculadaes_ES
dc.contributor.authorPérez-Pérez, María Estheres_ES
dc.contributor.authorMartínez-Force, Enriquees_ES
dc.contributor.authorKim, Hee-Sikes_ES
dc.contributor.authorHe, Yonghuaes_ES
dc.contributor.authorUmen, James G.es_ES
dc.contributor.authorCrespo, José L.es_ES
dc.date.accessioned2018-04-23T07:01:32Z-
dc.date.available2018-04-23T07:01:32Z-
dc.date.issued2018-
dc.identifier.citationJournal of Experimental Biology, 69(6): 1355-1367 (2018)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/163960-
dc.description.abstractAutophagy is an intracellular catabolic process that allows cells to recycle unneeded or damaged material to maintain cellular homeostasis. This highly dynamic process is characterized by the formation of double-membrane vesicles called autophagosomes, which engulf and deliver the cargo to the vacuole. Flow of material through the autophagy pathway and its degradation in the vacuole is known as autophagic flux, and reflects the autophagic degradation activity. A number of assays have been developed to determine autophagic flux in yeasts, mammals, and plants, but it has not been examined yet in algae. Here we analyzed autophagic flux in the model green alga Chlamydomonas reinhardtii. By monitoring specific autophagy markers such as ATG8 lipidation and using immunofluorescence and electron microscopy techniques, we show that concanamycin A, a vacuolar ATPase inhibitor, blocks autophagic flux in Chlamydomonas. Our results revealed that vacuolar lytic function is needed for the synthesis of triacylglycerols and the formation of lipid bodies in nitrogen- or phosphate-starved cells. Moreover, we found that concanamycin A treatment prevented the degradation of ribosomal proteins RPS6 and RPL37 under nitrogen or phosphate deprivation. These results indicate that autophagy might play an important role in the regulation of lipid metabolism and the recycling of ribosomal proteins under nutrient limitation in Chlamydomonases_ES
dc.language.isoenges_ES
dc.publisherOxford University Presses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectAutophagyes_ES
dc.subjectChlamydomonases_ES
dc.subjectConcanamycin A,es_ES
dc.subjectLipid bodyes_ES
dc.subjectNitrogenes_ES
dc.subjectPhosphatees_ES
dc.subjectRibosomal proteines_ES
dc.subjectTriacylglyceroles_ES
dc.titleAutophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonases_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1093/jxb/erx372-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhtpp://dx.doi.org/10.1093/jxb/erx372es_ES
dc.rights.license(http://creativecommons.org/licenses/by/4.0/),es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
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