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Role of IGF-1 as regulator of autophagy during otic vesicle development

AuthorsPulido, Sara; Magariños, Marta ; León, Yolanda; Varela-Nieto, Isabel
Issue Date2017
CitationFEBS3+ (2017)
XL SEBBM Congress (2017)
AbstractOtic vesicle development involves the coordinated actions of apoptosis, proliferation, senescence, cell diff erentiation and autophagy. Insulin-like growth factor-1 (IGF-1) is a key element in chicken otic development, and its defi cit causes syndromic deafness in mice and men. Autophagy is a catabolic process essential for vertebrate development and homeostasis. Our previous work showed that autophagy is required to facilitate the clearance of apoptotic otic cells and for proper acoustic-vestibular ganglion (AVG) development. Here we have studied the role of IGF-1 as a potential modulator of autophagy during otic development in chicken otocyst cultures. Ex vivo cultures of chicken otocysts were pre-treated with IGF1R inhibitors (PPP, NVP-AEW541) and stimulated with exogenous IGF-1. Bafi lomycin A1 was used to inhibit the fusion of autophagosomes to lysosomes. The modulation of signalling pathways was confi rmed by Western blotting of typical IGF-1 targets and of autophagic-LC3II relative levels. Proliferation and apoptosis were assessed by detection of mitotic-PH3, incorporation of EdU and TUNEL labelling. Otic neurogenesis was studied by immunofluorescence of neural markers. Our results show that LC3II relative levels were reduced by IGF-1. The accumulation of apoptotic cells observed in autophagy-inhibited otic vesicles was not ameliorated by IGF-1, however proliferation defects were partially reverted. AVG generation was completely blocked in autophagy-inhibited otic vesicles treated with IGF-1. Taken together, these results show that IGF-1 negatively regulates autophagy during early otic development.
DescriptionResumen del póster presentado al 1st Joint Meeting of the French-Portuguese-Spanish Biochemical and Molecular Biology Societies y al XL Spanish Society of Biochemistry and Molecular Biology (SEBBM) Congress, celebrado en Barcelona (España) del 23 al 26 de octubre de 2017.
Appears in Collections:(IIBM) Comunicaciones congresos
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