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From young to adult: in vivo monitoring of Ca2+ dynamics in C. elegans pharynx

AuthorsAlvarez-Illera, Pilar; García-Casas, Paloma; Arias-del-Val, Jessica; Fonteriz, Rosalba I. CSIC ORCID; Álvarez, Javier; Montero, Mayte CSIC ORCID CVN
Issue Date2017
CitationVI Spanish Worm Meeting (2017)
AbstractLifespan seems to be strongly associated to metabolic changes in C. elegans. To understand aging, it is therefore very important to define functional parameters able to report information on their metabolic state and energy balance. Intracellular Ca2+is a key signal transducer for muscle contraction, energy production and metabolic activation. We have monitored intracellular Ca2+-dynamics in C. elegans pharynx muscle in vivo along aging. Our data revealed surprisingly complex and age-dependent Ca2+patterns. Despite the progressive decline of pharynx pumping with age, we observed unaltered fast Ca2+ oscillations both in young and old worms. In addition, sporadic prolonged Ca2+increases lasting many seconds or minutes were often observed in between periods of fast Ca2+ oscillations. We attribute them to the inhibition of ATP-dependent Ca2+-pumps upon energy depletion. Accordingly, food deprivation largely augmented the frequency of prolonged [Ca2+] increases. However, paradoxically, prolonged [Ca2+] increases were more frequently observed in young worms than in older ones, and less frequently observed in energy-deficient mitochondrial respiratory chain nuo-6 mutants than in wild-type controls. We hypothesize that young animals are more susceptible to energy depletion due to their faster energy consumption rate, while nuo-6 mutants may keep better the energy balance by slowing energy consumption. Our data therefore suggest that the metabolic state of the pharynx during feeding stimulation depends mainly on the delicate balance between the instant rates of energy production and consumption. Thus, in vivo monitoring of muscle Ca2+ dynamics can be used as a novel tool to study cellular energy availability.
DescriptionResumen del trabajo presentado al VI Spanish Worm Meeting, celebrado en Valencia del 9 al 10 de marzo de 2017.
Appears in Collections:(IBGM) Comunicaciones congresos
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