English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/163892
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

C. elegans THSC complex deficiency leads to replication stress and genome instability

AutorGarcía-Muse, Tatiana ; Zheleva, Angelina; Galindo, Ulises; Aguilera, Andrés
Fecha de publicación2017
CitaciónVI Spanish Worm Meeting (2017)
ResumenThe THSC/TREX-2 complex is conserved from yeast to humans and is involved in mRNP biogenesis. Defects in the coupling of transcription with mRNA processing and export results in DNA damage and genome instability from yeast to human cells. Notably, the genetic instability and transcriptional defects observed in yeast THSC mutants is due to R-loops accumulation as co-transcriptional cleavage of the transcript reduced to some extend both defects. However, recently our laboratory has show that depletion of human counterpart, despite increasing genomic instability, does not lead to an obvious accumulation of R-loops. Nevertheless, we decide to use the simple metazoan Caernorhabditis elegans to further address this unexpected data. Through the characterization of two putative members of the C. elegans THSC complex we have observed that lack of the THSC/TREX-2 THP-1 leads to, DNA damage accumulation and replication defects at the germline. Importantly, microinjection of RNase H, that removes R-loops, partially restores the Cy3-dUTP incorporation in the nematode germline consistent with R-loops interfering with replication progression. This research provides new insight into the THSC/TREX-2 complex contribution to the maintenance of genome integrity in multicellular organisms.
DescripciónResumen del trabajo presentado al VI Spanish Worm Meeting, celebrado en Valencia del 9 al 10 de marzo de 2017.
URIhttp://hdl.handle.net/10261/163892
Aparece en las colecciones: (CABIMER) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.