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Título: | ATP synthesis coupled to the electroenzymatic activity of a hydrogenase immobilized at an electrode/biomimetic membrane interface |
Autor: | López de Lacey, Antonio CSIC ORCID ; Gutiérrez-Sanz, Óscar CSIC ORCID; Natale, Paolo; Márquez, Ileana F. CSIC; Marques, Marta C.; Zacarias, Sonia; Pita, Marcos CSIC ORCID ; Pereira, Inês A. C.; López-Montero, Iván; Vélez, Marisela CSIC ORCID | Fecha de publicación: | jul-2017 | Citación: | XXIV International Symposium on Bioelectrochemistry and Bioenergetics (2017) | Resumen: | Cells generate energy by coupling a proton gradient across a phospholipid bilayer membrane with the activity of a cross-membrane ATP synthase enzyme. In an effort to mimic this process in an artificial environment, we show that ATP can be efficiently produced starting from molecular hydrogen as a fuel. The proton concentration in an electrode/phospholipid bilayer interface can be controlled and monitorised electrochemically by immobilizing the membrane-bound [NiFeSe]-hydrogenase from Desulfovibrio vulgaris Hildenborough.1 The electro-enzymatic oxidation of H2 generated a proton gradient across the supported biomimetic membrane that can be coupled to the in vitro synthesis of ATP by reconstituting ATP-synthase from E. coli on the biomimetic system.2 | Descripción: | Trabajo presentado en el XXIV International Symposium on Bioelectrochemistry and Bioenergetics (BES 2017), celebrado en Lyon del 3 al 7 de julio de 2017. | URI: | http://hdl.handle.net/10261/163874 |
Aparece en las colecciones: | (ICP) Comunicaciones congresos |
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ATP_synthesis_Lacey.pdf | 1,57 MB | Adobe PDF | Visualizar/Abrir |
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