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Title: | ATP Synthesis and Biosensing Coupled to the Electroenzymatic Activity of a Hydrogenase on an Electrode/Biomimetic Membrane Interface |
Authors: | Pita, Marcos ![]() ![]() ![]() ![]() ![]() |
Issue Date: | 29-Nov-2017 |
Publisher: | Multidisciplinary Digital Publishing Institute |
Citation: | Proceedings 1(8): 766 (2017) |
Abstract: | Cells generate energy by coupling a proton gradient across a phospholipid bilayer membrane with the activity of a cross-membrane ATP synthase enzyme. In an effort to mimic this process in an artificial environment, we show that ATP can be efficiently produced starting from molecular hydrogen as a fuel. The proton concentration in an electrode/phospholipid bilayer interface can be controlled and monitorised electrochemically by immobilizing the membrane-bound [NiFeSe]- hydrogenase from Desulfovibrio vulgaris Hildenborough [1]. The electro-enzymatic oxidation of H2 generated a proton gradient across the supported biomimetic membrane that can be coupled to the in vitro synthesis of ATP by reconstituting ATP-synthase from E. coli on the biomimetic system [2]. Such a system is also suitable for developing an electrochemical biosensor of ATP. |
Description: | This article belongs to the Proceedings of the 5th International Symposium on Sensor Science (I3S 2017). |
Publisher version (URL): | http://dx.doi.org/10.3390/proceedings1080766 |
URI: | http://hdl.handle.net/10261/163825 |
DOI: | 10.3390/proceedings1080766 |
ISSN: | 2504-3900) |
Appears in Collections: | (ICP) Artículos |
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membrane_interface_Pita.pdf | 140,16 kB | Adobe PDF | ![]() View/Open |
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