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ATP Synthesis and Biosensing Coupled to the Electroenzymatic Activity of a Hydrogenase on an Electrode/Biomimetic Membrane Interface

AuthorsPita, Marcos ; Gutiérrez Sánchez, Cristina ; Natale, Paolo; García-Molina, Gabriel; Márquez, Ileana F. ; Marques, Marta C.; Zacarias, Sonia; Pereira, Inês A. C.; López-Montero, Iván; Vélez, Marisela ; López de Lacey, Antonio
Issue Date29-Nov-2017
PublisherMultidisciplinary Digital Publishing Institute
CitationProceedings 1(8): 766 (2017)
AbstractCells generate energy by coupling a proton gradient across a phospholipid bilayer membrane with the activity of a cross-membrane ATP synthase enzyme. In an effort to mimic this process in an artificial environment, we show that ATP can be efficiently produced starting from molecular hydrogen as a fuel. The proton concentration in an electrode/phospholipid bilayer interface can be controlled and monitorised electrochemically by immobilizing the membrane-bound [NiFeSe]- hydrogenase from Desulfovibrio vulgaris Hildenborough [1]. The electro-enzymatic oxidation of H2 generated a proton gradient across the supported biomimetic membrane that can be coupled to the in vitro synthesis of ATP by reconstituting ATP-synthase from E. coli on the biomimetic system [2]. Such a system is also suitable for developing an electrochemical biosensor of ATP.
DescriptionThis article belongs to the Proceedings of the 5th International Symposium on Sensor Science (I3S 2017).
Publisher version (URL)http://dx.doi.org/10.3390/proceedings1080766
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