English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/163795
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Synthesis of cobalt ferrite core/metallic shell nanoparticles for the development of a specific PNA/DNA biosensor

AuthorsPita, Marcos ; Abad, José M.; Vaz Domínguez, C. ; Briones, Carlos ; Mateo-Martí, Eva ; Martín-Gago, José A. ; Morales, M. P. ; Fernández López, Víctor Manuel
KeywordsMagnetic nanoparticle
Gold shell
Peptide nucleic acid
DNA–PNA hybridization
Mutation detection
Rhodamine 6G
Issue Date15-May-2008
PublisherElsevier
CitationJournal of Colloid and Interface Science 321(2): 484-492 (2008)
AbstractControlled synthesis of cobalt ferrite superparamagnetic nanoparticles covered with a gold shell has been achieved by an affinity and trap strategy. Magnetic nanoparticles are functionalized with a mixture of amino and thiol groups that facilitate the electrostatic attraction and further chemisorption of gold nanoparticles, respectively. Using these nanoparticles as seeds, a complete coating shell is achieved by gold salt-iterative reduction leading to monodisperse water-soluble gold-covered magnetic nanoparticles, with an average diameter ranging from 21 to 29 nm. These constitute a versatile platform for immobilization of biomolecules via thiol chemistry, which is exemplified by the immobilization of peptide nucleic acid (PNA) oligomers that specifically hybridize with complementary DNA molecules in solution. Hybridation with DNA probes has been measured using Rhodamine 6G fluorescence marker and the detection of a single nucleotide mutation has been achieved. These results suggest the PNA-nanoparticles application as a biosensor for DNA genotyping avoiding commonly time-consuming procedures employed.
Publisher version (URL)https://doi.org/10.1016/j.jcis.2008.02.010
URIhttp://hdl.handle.net/10261/163795
DOI10.1016/j.jcis.2008.02.010
ISSN0021-9797
Appears in Collections:(ICP) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.