English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/163795
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Synthesis of cobalt ferrite core/metallic shell nanoparticles for the development of a specific PNA/DNA biosensor

AutorPita, Marcos ; Abad, José M.; Vaz-Domínguez, Cristina; Briones, Carlos ; Mateo-Martí, Eva ; Martín-Gago, José A. ; Morales, M. P. ; Fernández López, Víctor Manuel
Palabras claveMagnetic nanoparticle
Gold shell
Peptide nucleic acid
DNA–PNA hybridization
Mutation detection
Rhodamine 6G
Fecha de publicación15-may-2008
EditorElsevier
CitaciónJournal of Colloid and Interface Science 321(2): 484-492 (2008)
ResumenControlled synthesis of cobalt ferrite superparamagnetic nanoparticles covered with a gold shell has been achieved by an affinity and trap strategy. Magnetic nanoparticles are functionalized with a mixture of amino and thiol groups that facilitate the electrostatic attraction and further chemisorption of gold nanoparticles, respectively. Using these nanoparticles as seeds, a complete coating shell is achieved by gold salt-iterative reduction leading to monodisperse water-soluble gold-covered magnetic nanoparticles, with an average diameter ranging from 21 to 29 nm. These constitute a versatile platform for immobilization of biomolecules via thiol chemistry, which is exemplified by the immobilization of peptide nucleic acid (PNA) oligomers that specifically hybridize with complementary DNA molecules in solution. Hybridation with DNA probes has been measured using Rhodamine 6G fluorescence marker and the detection of a single nucleotide mutation has been achieved. These results suggest the PNA-nanoparticles application as a biosensor for DNA genotyping avoiding commonly time-consuming procedures employed.
Versión del editorhttps://doi.org/10.1016/j.jcis.2008.02.010
URIhttp://hdl.handle.net/10261/163795
DOI10.1016/j.jcis.2008.02.010
ISSN0021-9797
Aparece en las colecciones: (ICP) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.