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Title

Generation of Vsx1/Vsx2 transcription factor double mutant in zebrafish using Crispr/cas9 system

AuthorsGonzález Díaz, Sergio; Letelier, Joaquin; Martínez-Morales, Juan Ramón
Issue Date2016
PublisherSociedad Española de Biología Celular
Citation11th Meeting SEBD (2016)
AbstractVisual system homeobox genes Vsx1 and Vsx2 are transcription factors that play an essential role in eye patterning and differentiation and have been linked to human diseases like microphthalmia/anophthalmia and coloboma. Taken advantage of the CRISPR/Cas9 system, we have generated several zebrafish lines with different deletions in the vsx1/vsx2 homeodomain, in order to obtain null mutations for phenotypic analysis. So far, we have generated 2 CRISPR lines for Vsx1 with deletions of 66bp and 245pb, both lacking most part of exon 3, which is the core DNA binding motif in vsx TFs. For vsx2 we generated 3 different alleles, two with small deletions in exon1 and exon 3 (8bp and 4bp, respectively) and one with a 73bp deletion in exon3. We found no clear developmental defects in vsx2E1Δ8bp maternal zygotic homozygous mutant, vsx2E3Δ4bp zygotic mutant and vsx1Δ66bp zygotic mutant. Currently, we are analyzing if compensatory changes in vsx1/vsx2 gene expression can explain the lack of morphological phenotype observed. In addition, we are crossing both vsx1 and vsx2 alleles in order to generate the double mutant, which will be used as a tool to investigate the impact of vsx paralogs in the gene regulatory network involved in the specification of the neural retina domain.
DescriptionResumen del póster presentado al 11th Meeting of the Spanish Society for Developmental Biology, celebrado en Girona (España) del 19 al 21 de octubre de 2016.
URIhttp://hdl.handle.net/10261/163778
Appears in Collections:(CABD) Comunicaciones congresos
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