English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/163778
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Generation of Vsx1/Vsx2 transcription factor double mutant in zebrafish using Crispr/cas9 system

AutorGonzález Díaz, Sergio; Letelier, Joaquin; Martínez-Morales, Juan Ramón
Fecha de publicación2016
EditorSociedad Española de Biología Celular
Citación11th Meeting SEBD (2016)
ResumenVisual system homeobox genes Vsx1 and Vsx2 are transcription factors that play an essential role in eye patterning and differentiation and have been linked to human diseases like microphthalmia/anophthalmia and coloboma. Taken advantage of the CRISPR/Cas9 system, we have generated several zebrafish lines with different deletions in the vsx1/vsx2 homeodomain, in order to obtain null mutations for phenotypic analysis. So far, we have generated 2 CRISPR lines for Vsx1 with deletions of 66bp and 245pb, both lacking most part of exon 3, which is the core DNA binding motif in vsx TFs. For vsx2 we generated 3 different alleles, two with small deletions in exon1 and exon 3 (8bp and 4bp, respectively) and one with a 73bp deletion in exon3. We found no clear developmental defects in vsx2E1Δ8bp maternal zygotic homozygous mutant, vsx2E3Δ4bp zygotic mutant and vsx1Δ66bp zygotic mutant. Currently, we are analyzing if compensatory changes in vsx1/vsx2 gene expression can explain the lack of morphological phenotype observed. In addition, we are crossing both vsx1 and vsx2 alleles in order to generate the double mutant, which will be used as a tool to investigate the impact of vsx paralogs in the gene regulatory network involved in the specification of the neural retina domain.
DescripciónResumen del póster presentado al 11th Meeting of the Spanish Society for Developmental Biology, celebrado en Girona (España) del 19 al 21 de octubre de 2016.
URIhttp://hdl.handle.net/10261/163778
Aparece en las colecciones: (CABD) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.