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Title

3D chromatin organisation and enhancer-promoter specificity

AuthorsVicente-García, Cristina; López-Mayorga, Macarena ; Irastorza-Azcárate, Ibai; Tena, Juan J. ; Devos, Damien P.; Gómez-Skarmeta, José Luis ; Carvajal, Jaime J.
Issue Date2016
PublisherSociedad Española de Biología Celular
Citation11th Meeting SEBD (2016)
AbstractMyogenic regulatory factors (MRFs) control the determination, specification and differentiation of skeletal muscle during development. Two of these factors, Myf5 and Mrf4, are closely linked in the genome of all vertebrates analyzed. Their transcriptional regulation relies on multiple enhancers that are intermingled and scattered throughout the locus. In this work, we try to unveil the mechanisms by which precise long-range interactions between enhancers and promoters are established; interactions that change during development and differentiation in order to allow the genes to be accurately expressed according to their highly specific spatiotemporal expression pattern. We hypothesize the existence of a novel type of regulatory element named TRABS (transcriptional balancing sequences) which, together with the promoters and enhancers of the locus, create a series of equilibria states that ensure the establishment of the correct enhancer-promoter regulatory interactions. To reveal the three-dimensional organization of the locus, 4C experiments were carried out in the muscle-derived cell line C2C12 both in growing and differentiating conditions, as well in embryos at different developmental stages and genotypes (including KOs for Mrf4 or Myf5 promoters and TRABS). The integration of all the generated datasets would improve our understanding of how this locus is organized and regulated during the formation of skeletal muscle throughout development.
DescriptionResumen del póster presentado al 11th Meeting of the Spanish Society for Developmental Biology, celebrado en Girona (España) del 19 al 21 de octubre de 2016.
URIhttp://hdl.handle.net/10261/163734
Appears in Collections:(CABD) Comunicaciones congresos
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