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Mutations of genes involved in the early-eye morphogenesis in zebrafish

AutorUndurraga, Cristian; Letelier, Joaquin; Vázquez-Marín, Javier; Martínez-Morales, Juan Ramón
Fecha de publicación2016
EditorSociedad Española de Biología Celular
Citación11th Meeting SEBD (2016)
ResumenThe organogenesis of vertebrate eye is a process that requires a complex choreography between inductive signals, intrinsic genetic programs and morphogenetic movements. All these process finally result in a three dimensional organ comprising functionally specialized tissues. Two of these tissues are the neural retina (NR) and the retinal pigmented epithelium (RPE), which are specified in the developmental window comprised between the establishment of the eye field and the onset of neuronal differentiation. The vertebrate fish zebrafish (Danio rerio) and medaka (Oryzias latipes) are excellent models to observe the eye development at early stages also to do reverse genetic to investigate gene function. We performed on zebrafish a RNA-seq assay to identify genes that can act as direct morphogenetic effectors during early eye development. Using the latest technology CRISPR/Cas9, we selected 3 genes to generate loss of function models in zebrafish: rac3b, rab32a and lamtor3, and 2 genes to generate loss of function alleles both in zebrafish and medaka: supervillin-a and supervillin- b. For all these genes we have generated Indels mutations, and homozygous mutants lines are now available for rac3b, supervillin-a and supervillin-b. We did not observe any evident embryonic phenotype in these homozygous mutants. We are currently investigating which compensatory mechanisms may account for the apparently normal phenotypes observed.
DescripciónResumen del póster presentado al 11th Meeting of the Spanish Society for Developmental Biology, celebrado en Girona (España) del 19 al 21 de octubre de 2016.
URIhttp://hdl.handle.net/10261/163727
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