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Title: | Immobilization of Redox Enzymes on Nanoporous Gold Electrodes: Applications in Biofuel Cells |
Authors: | Siepenkoetter, Till; Salaj‐Kosla, Urszula; Xiao, Xinxin; Ó Conghaile, Peter; Pita, Marcos ![]() |
Issue Date: | Apr-2017 |
Publisher: | Wiley-VCH |
Citation: | ChemPlusChem 82(4): 553-560 (2017) |
Abstract: | Nanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold:silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FAD‐dependent glucose dehydrogenase (GDH) (Glomorella cingulata) and bilirubin oxidase (BOx) (Myrothecium verrucaria). Glucose dehydrogenase was immobilized by drop‐casting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazonium‐modified NPG electrode. The stability of the bilirubin‐oxidase‐modified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOx‐modified NPG cathode (500 nm thickness) and a GDH‐modified anode (300 nm), which generated power densities of 17.5 and 7.0 μW cm−2 in phosphate‐buffered saline and artificial serum, respectively. |
Publisher version (URL): | https://doi.org/10.1002/cplu.201600455 |
URI: | http://hdl.handle.net/10261/163726 |
DOI: | 10.1002/cplu.201600455 |
E-ISSN: | 2192-6506 |
Appears in Collections: | (ICP) Artículos |
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