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Immobilization of Redox Enzymes on Nanoporous Gold Electrodes: Applications in Biofuel Cells

AuthorsSiepenkoetter, Till; Salaj‐Kosla, Urszula; Xiao, Xinxin; Ó Conghaile, Peter; Pita, Marcos ; Ludwig, Roland; Magner, Edmond
Issue DateApr-2017
CitationChemPlusChem 82(4): 553-560 (2017)
AbstractNanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold:silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FAD‐dependent glucose dehydrogenase (GDH) (Glomorella cingulata) and bilirubin oxidase (BOx) (Myrothecium verrucaria). Glucose dehydrogenase was immobilized by drop‐casting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazonium‐modified NPG electrode. The stability of the bilirubin‐oxidase‐modified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOx‐modified NPG cathode (500 nm thickness) and a GDH‐modified anode (300 nm), which generated power densities of 17.5 and 7.0 μW cm−2 in phosphate‐buffered saline and artificial serum, respectively.
Publisher version (URL)https://doi.org/10.1002/cplu.201600455
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