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Título

The emergence of the rac3b/rfng/sgca synexpression group in the ostariophysi superorder refined the mechanisms responsible for rhombomere segregation during hindbrain development

AutorLetelier, Joaquin; Avila, Carlos; Maeso, Ignacio; Gómez-Skarmeta, José Luis ; Pujades, Cristina; Martínez-Morales, Juan Ramón
Fecha de publicación2016
EditorSociedad Española de Biología Celular
Citación11th Meeting SEBD (2016)
ResumenCompartmentalization of cells during development is a fundamental process that allows the segregation of precursor populations into distinct tissue domains. The vertebrate hindbrain (HB) is subdivided along the antero-posterior axis in 7 rhombomeres (Rs) emerging during development through a finely tuned cascade of TFs that confer identity to each R. In zebrafish, each rhombomere is flanked by actomyosin cables that prevent cells to intermingle. These cables form physical boundaries that keep neuroblasts confined to their precise location within the HB. Here we found that the small GTPase Rac3b is expressed at the HB boundaries and regulate actomyosin cables assembly. In addition, Rac3b was found to be part of a synexpression group of genes only found in teleost fish from the Ostariophysi superorder, a group that comprise 68% of fresh water species. Comparative genomics and chromosomal conformation capture analysis suggest that after the teleost WGD, the novel expression pattern emerged in the Ostariophysi lineage by a chromosomal inversion causing the fusion of two highly conserved TADs. Using an enhancer reporter vector we found two CREs that drive expression to R boundaries. A full deletion (Δ2,5kb) of both enhancers was generated using the CRISPR/Cas9 system. Phenotypic analysis of this mutant points to the existence of additional CREs (i.e. shadow enhancers) within the novel TAD directing the expression of these genes to the boundaries. The observed redundancy stresses the relevance of this novel mechanism for rhombomere segregation during development.
DescripciónResumen del trabajo presentado al 11th Meeting of the Spanish Society for Developmental Biology, celebrado en Girona (España) del 19 al 21 de octubre de 2016.-- et al.
URIhttp://hdl.handle.net/10261/163716
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