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Unraveling plant cellular targets for the Rhizobium-specific effectors NopL and NopP

AuthorsJiménez-Guerrero, Irene; Pérez-Montaño, Francisco; Medina, Carlos ; Santero, Eduardo ; Monreal, J. A.; Ollero, F. J.; López-Baena, Francisco Javier
Issue Date2016
Citation12th European Nitrogen Fixation Conference (2016)
AbstractPathogenic Gram-negative use a specialized apparatus called Type 3 secretion system (T3SS) to deliver effectors directly into the eukaryotic host cells. These effectors suppress plant defenses to promote disease but they can also be recognized by specific plant receptors that trigger a strong defense reaction to eliminate the pathogen. The T3SS has also been found in some symbiotic rhizobial strains and the effectors secreted are involved in host-range determination and symbiotic efficiency. The broad host-range bacterium S. fredii HH103 secretes two proteins through the T3SS, NopL and NopP, which are specific for rhizobia. In this work we studied the function of both effectors in the symbiosis with soybean, which is considered its natural host plant. NopL and NopP were phosphorylated by soybean root kinases and the phosphorylation cascade was Ca2+ - and calmodulin-dependent. While the signaling pathway that culminates in the phosphorylation of NopL included ser/thr and MAPKK kinases, in the case of NopP this pathway involved ser/thr and tyr kinases but not MAPKK kinases. Transient expression of both nopL and nopP fused to YFP in Nicotiana benthamiana leaves and further confocal imaging indicated that they localized to the nucleus of the host cell. The use of a yeast-based array to determine possible effectors functions indicated that NopP could be involved in nuclear localization and migration. Finally, co-immunoprecipitation analyses of N. benthamiana NopL- and NopP-interacting proteins and further mass spectrometry analyses identified several potential plant targets for these effectors. The most interesting interactions are currently being validated by Bimolecfular Fluorescence Complementation (BiFC).
DescriptionResumen del póster presentado al 12th European Nitrogen Fixation Conference, celebrado en Budapest (Hungria) del 25 al 28 de agosto de 2016.
Appears in Collections:(CABD) Comunicaciones congresos
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