English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/163688
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Evolutionary emergence of the rac3b/rfng/sgca regulatory cluster refined mechanisms for hindbrain boundaries formation

AutorLetelier, Joaquin; Undurraga, Cristian; Polvillo Hernández, Rocío; Tena, Juan J. ; Maeso, Ignacio; Gómez-Skarmeta, José Luis ; Martínez-Morales, Juan Ramón ; Pujades, Cristina
Palabras claveHindbrain boundaries
Regulatory landscape
Segmentation
cis-regulatory elements
Rhombomeres
Fecha de publicación2018
EditorNational Academy of Sciences (U.S.)
CitaciónProceedings of the National Academy of Sciences: 201719885 (2018)
ResumenDevelopmental programs often rely on parallel morphogenetic mechanisms that guarantee precise tissue architecture. While redundancy constitutes an obvious selective advantage, little is known on how novel morphogenetic mechanisms emerge during evolution. In zebrafish, rhombomeric boundaries behave as an elastic barrier, preventing cell intermingling between adjacent compartments. Here, we identify the fundamental role of the small-GTPase Rac3b in actomyosin cable assembly at hindbrain boundaries. We show that the novel rac3b/rfng/sgca regulatory cluster, which is specifically expressed at the boundaries, emerged in the Ostariophysi superorder by chromosomal rearrangement that generated new cis-regulatory interactions. By combining 4C-seq, ATAC-seq, transgenesis, and CRISPR-induced deletions, we characterized this regulatory domain, identifying hindbrain boundary-specific cis-regulatory elements. Our results suggest that the capacity of boundaries to act as an elastic mesh for segregating rhombomeric cells evolved by cooption of critical genes to a novel regulatory block, refining the mechanisms for hindbrain segmentation.
DescripciónLetelier, Joaquin et al.
Versión del editorhttps://doi.org/10.1073/pnas.1719885115
URIhttp://hdl.handle.net/10261/163688
DOI10.1073/pnas.1719885115
ISSN0027-8424
E-ISSN1091-6490
Aparece en las colecciones: (CABD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
rac3b-rfng-sgca.pdf18,3 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.