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Title

Analytical Validation of a New Enzymatic and Automatable Method for d-Xylose Measurement in Human Urine Samples

AuthorsSánchez-Moreno, Israel ; Monsalve-Hernando, C.; Godino, A.; Illa, L.; Gaspar, M. J.; Muñoz, G. M.; Díaz, A.; Martín, J. L.; García-Junceda, Eduardo ; Fernández-Mayoralas, Alfonso ; Hermida, Carmen
Issue Date2017
PublisherHindawi Publishing Corporation
CitationBioMed Research International 2017 (2017)
AbstractHypolactasia, or intestinal lactase deficiency, affects more than half of the world population. Currently, xylose quantification in urine after gaxilose oral administration for the noninvasive diagnosis of hypolactasia is performed with the hand-operated nonautomatable phloroglucinol reaction. This work demonstrates that a new enzymatic xylose quantification method, based on the activity of xylose dehydrogenase from Caulobacter crescentus, represents an excellent alternative to the manual phloroglucinol reaction. The new method is automatable and facilitates the use of the gaxilose test for hypolactasia diagnosis in the clinical practice. The analytical validation of the new technique was performed in three different autoanalyzers, using buffer or urine samples spiked with different xylose concentrations. For the comparison between the phloroglucinol and the enzymatic assays, 224 urine samples of patients to whom the gaxilose test had been prescribed were assayed by both methods. A mean bias of -16.08 mg of xylose was observed when comparing the results obtained by both techniques. After adjusting the cut-off of the enzymatic method to 19.18 mg of xylose, the Kappa coefficient was found to be 0.9531, indicating an excellent level of agreement between both analytical procedures. This new assay represents the first automatable enzymatic technique validated for xylose quantification in urine.
Publisher version (URL)http://dx.doi.org/10.1155/2017/8421418
URIhttp://hdl.handle.net/10261/163673
Identifiersdoi: 10.1155/2017/8421418
issn: 2314-6133
e-issn: 2314-6141
Appears in Collections:(IQOG) Artículos
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