English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/163670
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Two coffins and a funeral: early or late caspase activation determines two types of apoptosis induced by DNA damaging agents

AutorOropesa-Ávila, Manuel; Cruz, Patricia de la; Porcuna, Jesús; Villanueva Paz, Marina; Fernández-Vega, Alejandro; Mata, Mario de la ; Lavera, I. de; Suarez-Rivero, Juan M.; Luzón-Hidalgo, Raquel; Álvarez-Córdoba, Mónica; Cotán, David ; Zaderenko, Paula; Cordero, Mario D. ; Sánchez-Alcázar, José Antonio
Palabras claveCamptothecin
Caspases
Actin
Microtubules
Apoptosis
Fecha de publicación2017
EditorSpringer Nature
CitaciónApoptosis 22(3): 421-436 (2017)
ResumenCell cytoskeleton makes profound changes during apoptosis including the organization of an Apoptotic Microtubule Network (AMN). AMN forms a cortical structure which plays an important role in preserving plasma membrane integrity during apoptosis. Here, we examined the cytoskeleton rearrangements during apoptosis induced by camptothecin (CPT), a topoisomerase I inhibitor, in human H460 and porcine LLCPK-1α cells. Using fixed and living cell imaging, we showed that CPT induced two dose- and cell cycle-dependent types of apoptosis characterized by different cytoskeleton reorganizations, time-dependent caspase activation and final apoptotic cell morphology. In the one referred as “slow” (~h) or round-shaped, apoptosis was characterized by a slow contraction of the actinomyosin ring and late caspase activation. In “slow” apoptosis the γ-tubulin complexes were not disorganized and microtubules were not depolymerized at early stages. In contrast, “fast” (~min) or irregular-shaped apoptosis was characterized by early caspase activation followed by full contraction of the actinomyosin ring. In fast apoptosis γ-tubulin complexes were disorganized and microtubules were initially depolymerized. However, after actinomyosin contraction, microtubules were reformed adopting a cortical but irregular disposition near plasma membrane. In addition to distinctive cytoskeleton reorganization kinetics, round and irregular-shaped apoptosis showed different biological properties with respect to AMN maintenance, plasma membrane integrity and phagocytes response. Our results suggest that the knowledge and modulation of the type of apoptosis promoted by genotoxic agents may be important for deciding a better therapeutic option and predicting the immune response in cancer treatment.
URIhttp://hdl.handle.net/10261/163670
Identificadoresdoi: 10.1007/s10495-016-1337-z
e-issn: 1573-675X
issn: 1360-8185
Aparece en las colecciones: (CABD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.