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Title

Oxygen biosensor based on bilirubin oxidase immobilized on a nanostructured gold electrode

AuthorsPita, Marcos ; Gutiérrez Sánchez, Cristina ; Toscano, Miguel D.; Shleev, Sergey; López de Lacey, Antonio
KeywordsBiocatalysis
Bilirubin oxidase
Biosensor
Direct electron transfer
Gold nanoparticles
Issue DateDec-2013
PublisherElsevier
CitationBioelectrochemistry 94: 69-74 (2013)
AbstractGold disk electrodes modified with gold nanoparticles have been used as a scaffold for the covalent immobilization of bilirubin oxidase. The nanostructured bioelectrodes were tested as mediator-less biosensors for oxygen in a buffer that mimics the content and the composition of human physiological fluids. Chronoamperometry measurements showed a detection limit towards oxygen of 6 ± 1 μM with a linear range of 6-300 μM, i.e. exceeding usual physiological ranges of oxygen in human tissues and fluids. The biosensor presented is the first ever-reported oxygen amperometric biosensor based on direct electron transfer of bilirubin oxidase.
Publisher version (URL)https://doi.org/10.1016/j.bioelechem.2013.07.001
URIhttp://hdl.handle.net/10261/163634
DOI10.1016/j.bioelechem.2013.07.001
ISSN1567-5394
E-ISSN1878-562X
Appears in Collections:(ICP) Artículos
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