Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/163551
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dc.contributor.authorRomero-Moya, Damià-
dc.contributor.authorCastaño, Julio-
dc.contributor.authorSantos-Ocaña, Carlos-
dc.contributor.authorNavas, Plácido-
dc.contributor.authorMenendez, Pablo-
dc.date.accessioned2018-04-12T10:20:40Z-
dc.date.available2018-04-12T10:20:40Z-
dc.date.issued2017-
dc.identifierdoi: 10.1016/j.scr.2016.09.007-
dc.identifiere-issn: 1876-7753-
dc.identifierissn: 1873-5061-
dc.identifier.citationStem Cell Research 24: 144-147 (2017)-
dc.identifier.urihttp://hdl.handle.net/10261/163551-
dc.description.abstractWe report the generation, CRISPR/Cas9-edition and characterization of induced pluripotent stem cell (iPSC) lines from a patient with coenzyme Q deficiency harboring the heterozygous mutation c.483G > C in the COQ4 gene. iPSCs were generated using non-integrative Sendai Viruses containing the reprogramming factors OCT4, SOX2, KLF4 and C-MYC. The iPSC lines carried the c.483G > C COQ4 mutation, silenced the OKSM expression and were mycoplasma-free. They were bona fide pluripotent cells as characterized by morphology, immunophenotype/gene expression for pluripotent-associated markers/genes, NANOG and OCT4 promoter demethylation, karyotype and teratoma formation. The COQ4 mutation was CRISPR/Cas9 edited resulting in isogenic, diploid and off-target free COQ4-corrected iPSCs.-
dc.publisherElsevier-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleGeneration, genome edition and characterization of iPSC lines from a patient with coenzyme Q10 deficiency harboring a heterozygous mutation in COQ4 gene-
dc.typeartículo-
dc.identifier.doi10.1016/j.scr.2016.09.007-
dc.relation.publisherversionhttps://doi.org/10.1016/j.scr.2016.09.007-
dc.date.updated2018-04-12T10:20:40Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.relation.csic-
dc.identifier.pmid28465093-
item.grantfulltextopen-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
item.openairetypeartículo-
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