English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/163473
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Characterization of a fetal liver cell population endowed with long-term multiorgan endothelial reconstitution potential

AutorCañete, Ana ; Comaills, Valentine ; Prados, Isabel ; Ybot, Patricia; Bockamp, Ernesto; Göttgens, Berthold; Sánchez, María José
Fecha de publicación2017
AlphaMed Press
CitaciónStem Cells 35(2): 507-521 (2017)
ResumenStable reconstitution of vascular endothelial beds upon transplantation of progenitor cells represents an important challenge due to the paucity and generally limited integration/expansion potential of most identified vascular related cell subsets. We previously showed that mouse fetal liver (FL) hemato/vascular cells from day 12 of gestation (E12), expressing the Stem Cell Leukaemia (SCL) gene enhancer transgene (SCL-PLAP cells), had robust endothelial engraftment potential when transferred to the blood stream of newborns or adult conditioned recipients, compared to the scarce vascular contribution of adult bone marrow cells. However, the specific SCL-PLAP hematopoietic or endothelial cell subset responsible for the long-term reconstituting endothelial cell (LTR-EC) activity and its confinement to FL developmental stages remained unknown. Using a busulfan-treated newborn transplantation model, we show that LTR-EC activity is restricted to the SCL-PLAPVE-cadherinCD45 cell population, devoid of hematopoietic reconstitution activity and largely composed by Lyve1 endothelial-committed cells. SCL-PLAP Ve-cadherinCD45 cells contributed to the liver sinusoidal endothelium and also to the heart, kidney and lung microvasculature. LTR-EC activity was detected at different stages of FL development, yet marginal activity was identified in the adult liver, revealing unknown functional differences between fetal and adult liver endothelial/endothelial progenitors. Importantly, the observations that expanding donor-derived vascular grafts colocalize with proliferating hepatocyte-like cells and participate in the systemic circulation, support their functional integration into young livers. These findings offer new insights into the engraftment, phonotypical, and developmental characterization of a novel endothelial/endothelial progenitor cell subtype with multiorgan LTR-EC activity, potentially instrumental for the treatment/genetic correction of vascular diseases.
Descripciónet al.
Versión del editorhttps://doi.org/10.1002/stem.2494
Identificadoresdoi: 10.1002/stem.2494
e-issn: 1549-4918
issn: 1066-5099
Aparece en las colecciones: (IBIS) Artículos
(CABD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
charapoten.pdf1,18 MBUnknownVisualizar/Abrir
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.