English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/163450
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


An efficient FLP-based toolkit for spatiotemporal control of gene expression in Caenorhabditis elegans

AuthorsMuñoz-Jiménez, Celia; Ayuso, Cristina CSIC ORCID; Dobrzynska, Agnieszka CSIC ORCID CVN; Torres-Méndez, Antonio; Cruz, Patricia de la; Askjaer, Peter CSIC ORCID
FLP-out gene inactivation
Tissue-specific gene expression
PEEL-1 toxin
Genome engineering
Issue Date2017
PublisherGenetics Society of America
CitationGenetics 206(4): 1763-1778 (2017)
AbstractSite-specific recombinases are potent tools to regulate gene expression. In particular, the Cre (cyclization recombination) and FLP (flipase) enzymes are widely used to either activate or inactivate genes in a precise spatiotemporal manner. Both recombinases work efficiently in the popular model organism Caenorhabditis elegans, but their use in this nematode is still only sporadic. To increase the utility of the FLP system in C. elegans, we have generated a series of single-copy transgenic strains that stably express an optimized version of FLP in specific tissues or by heat induction. We show that recombination efficiencies reach 100% in several cell types, such as muscles, intestine, and serotonin-producing neurons. Moreover, we demonstrate that most promoters drive recombination exclusively in the expected tissues. As examples of the potentials of the FLP lines, we describe novel tools for induced cell ablation by expression of the PEEL-1 toxin and a versatile FLP-out cassette for generation of GFP-tagged conditional knockout alleles. Together with other recombinase-based reagents created by the C. elegans community, this toolkit increases the possibilities for detailed analyses of specific biological processes at developmental stages inside intact animals.
Publisher version (URL)https://doi.org/10.1534/genetics.117.201012
Identifiersdoi: 10.1534/genetics.117.201012
issn: 0016-6731
e-issn: 1943-2631
Appears in Collections:(CABD) Artículos
Files in This Item:
File Description SizeFormat 
effiFLPelegan.pdf1,68 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.