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Title

Lamin binding proteins in Caenorhabditis elegans

AuthorsDobrzynska, Agnieszka ; Askjaer, Peter ; Gruenbaum, Yosef
KeywordsBAF-1
ChIP
CRISPR
DamID
FRET
Emerin
Lamin
FLIP
Issue Date2016
PublisherElsevier
CitationMethods in Enzymology 569: 455-483 (2016)
AbstractThe nuclear lamina, composed of lamins and numerous lamin-associated proteins, is required for mechanical stability, mechanosensing, chromatin organization, developmental gene regulation, mRNA transcription, DNA replication, nuclear assembly, and nuclear positioning. Mutations in lamins or lamin-binding proteins cause at least 18 distinct human diseases that affect specific tissues such as muscle, adipose, bone, nerve, or skin, and range from muscular dystrophies to lipodystrophy, peripheral neuropathy, or accelerated aging. Caenorhabditis elegans has unique advantages in studying lamin-binding proteins. These advantages include the low complexity of genes encoding lamin and lamin-binding proteins, advanced transgenic techniques, simple application of RNA interference, sophisticated genetic strategies, and a large collection of mutant lines. This chapter provides detailed and comprehensive protocols for the genetic and phenotypic analysis of lamin-binding proteins in C. elegans.
URIhttp://hdl.handle.net/10261/163333
Identifiersdoi: 10.1016/bs.mie.2015.08.036
issn: 0076-6879
e-issn: 1557-7988
Appears in Collections:(CABD) Artículos
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