English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/163168
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

A Pseudomonas putida cbrB transposon insertion mutant displays a biofilm hyperproducing phenotype that is resistant to dispersal

AuthorsAmador, Cristina I. ; López-Sánchez, Aroa ; Govantes, Fernando ; Santero, Eduardo ; Canosa, Inés
KeywordsBiofilm
Pseudomonas putida
CbrB
Environmental genomics
Issue Date2016
PublisherWiley-VCH
Society for Applied Microbiology
CitationEnvironmental Microbiology Reports 8(5): 622-629 (2016)
AbstractThe CbrAB two-component system in the Pseudomonads controls a variety of metabolic and behavioural traits required for its adaptation to changing environmental conditions, including the uptake or assimilation of certain carbon sources, and processes such as chemotaxis or stress tolerance. In this work we characterise a miniTn5-luxAB-Km transposon insertion mutant in cbrB (MPO406) in Pseudomonas putida leading to a biofilm overproducing phenotype that is not dispersed when nutrients are depleted. Comparison with a cbrB deletion mutant revealed that all phenotypes previously attributed to CbrB in P. putida correlated in both strains, with the exception of biofilm overproduction and absence of dispersal. We show that in the insertion mutant, the expression of the downstream regulatory RNA CrcZ is upregulated, and also show the presence of a truncated form of CbrB. Also, two additional point mutations in lapG and lapD have been detected in MPO406 by whole genome sequencing. Combination of these effects provides a robust biofilm overproducing phenotype. We present the mutant strain MPO406 as a good candidate to perform bio-production of substances of biotechnological interest or other processes such as bioremediation, which take advantage of immobilized cells on solid surfaces.
Publisher version (URL)https://doi.org/10.1111/1758-2229.12414
URIhttp://hdl.handle.net/10261/163168
Identifiersdoi: 10.1111/1758-2229.12414
e-issn: 1758-2229
Appears in Collections:(CABD) Artículos
Files in This Item:
File Description SizeFormat 
pseudisper.pdf1,8 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.