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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/163145
Título

α-Spectrin and integrins act together to regulate actomyosin and columnarization, and to maintain a monolayered follicular epithelium

AutorFu Ng, Bing; Kannan Selvaraj, Gokul; Santa-Cruz Mateos, Carmen; Grosheva, Inna; Alvarez-Garcia, Ines; Martín-Bermudo, María D. ; Palacios, Isabel M.
Palabras claveMonolayer
Epithelium
Proliferation
Cell shape
Tumor-like mass
Tissue architecture
Fecha de publicación2016
EditorCompany of Biologists
CitaciónDevelopment 143(8): 1388-1399 (2016)
ResumenThe Spectrin cytoskeleton crosslinks actin to the membrane, and although it has been greatly studied in erythrocytes, much is unknown about its function in epithelia. We have studied the role of Spectrins during epithelia morphogenesis using the Drosophila follicular epithelium (FE). As previously described, we show that α-Spectrin and β-Spectrin are essential to maintain a mono-layered FE, but, contrary to previous work, Spectrins are not required to control proliferation. Furthermore, spectrin cells show differentiation and polarity defects only in the ectopic layers of stratified epithelia, similar to integrin mutants. Our results identify α-Spectrin and integrins as novel regulators of apical constriction-independent cell elongation, as α-spectrin and integrin cells fail to columnarize. Finally, we show that increasing and reducing the activity of the Rho1-myosin-II pathway enhances and decreases multi-layering of α-spectrin cells, respectively. Similarly, higher myosin-II activity enhances the integrin multi-layering phenotype. This work identifies a primary role for α-Spectrin in controlling cell shape, perhaps by modulating actomyosin. All together, we suggest that a functional Spectrin-Integrin complex is essential to balance adequate forces, in order to maintain a mono-layered epithelium.
Versión del editorhttps://doi.org/10.1242/dev.130070
URIhttp://hdl.handle.net/10261/163145
Identificadoresdoi: 10.1242/dev.130070
e-issn: 1477-9129
issn: 0950-1991
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