English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/162834
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Dose-titration of virulent Neospora caninum isolate Nc-Spain7 in pregnant sheep at 90 days of gestation

AutorSánchez Sánchez, R.; Ferre, Ignacio; Re, M.; Ferrer, L. M.; Navarro Rodrigo, T.; Pizarro, M.; Tabanera, E.; Regidor-Cerrillo, Javier; Rico-San Román, L.; Callizo Esteve, N.; Benavides, Julio ; Ortega Mora, Luis M.
Fecha de publicación2017
EditorUniversidad Complutense de Madrid
Citación4th International Meeting on Apicomplexa in Farm Animals: 62 (2017)
ResumenNeospora caninum is considered one of the main causes of abortion in cattle and recent studies showed its relevance as abortifacient in small ruminants. Recently, the well-characterized NcSpain7 isolate has been tested in a pregnant sheep model of Neospora infection at mid gestation (90 days of gestation) using 106 tachyzoites intravenously and resulting in 100% abortion and highly parasite detection in target fetal tissues. This isolate has also been titrated in a pregnant mice model suggesting that a dose of 105 tachyzoites would be suitable for testing drugs or vaccine candidates. Thus, a refinement and standardization of the pregnant sheep model of neosporosis is also recommendable. Whit this purpose, pregnant sheep were inoculated intravenously at 90 days of gestation with 105 (n=6) (G1), 104 (n=5) (G2), 103 (n=5) (G4), 102 (n=4) (G5) tachyzoites; and subcutaneously with 104 tachyzoites (n=4) (G3) of the Nc-Spain7 or with PBS (n=3) (G6). Clinical outcome and lesions, parasite detection, parasite burden in target tissues and humoral and cellular immune responses were evaluated. Fetal mortality was detected between 32-44 days post infection in G1 (6 out of 6 pregnant ewes), G2 (4 out of 5 pregnant ewes), G3 (3 out of 4 pregnant ewes), G4 (3 out of 5 pregnant ewes), and G5 (2 out of 4 pregnant ewes). Pregnant sheep in G6 gave birth to healthy lambs. Significant differences were found in the fetal survival rate between G1 and G6 (P < 0.05). Serological analysis showed IFAT titers higher than 1:32 and 1:200 in thoracic liquid of all aborted fetuses and precolostral sera of all lambs, respectively. N. caninum DNA was widely detected in placentomes/cotyledons investigated from all animals in the different infected groups. In fetal brain, parasite DNA was detected in 81.8%, 91.7%, 87.5%, 66.7% and 90.5% of samples examined in G1, G2, G3, G4 and G5 respectively. Significant lower parasite detection was found in G4 compared to G2 (P < 0.05). Parasite load in fetal brain was lower in G2 (P < 0.05) and G4 (P < 0.001) compared to G1 and G5. In addition, G4 showed lower brain parasite burden compared to G2 (P < 0.05). In conclusion, clear differences were observed concerning abortion between pregnant sheep inoculated with different parasite doses while less pronounced differences related to vertical transmission to the fetus were found between doses or routes of administration.
DescripciónTrabajo presentado al: 4th International Meeting on Apicomplexa in Farm Animals. (Madrid, Spain. 11-14 October 2017).
Aparece en las colecciones: (IGM) Comunicaciones congresos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Embargo. Digital.CSIC..pdf21,67 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.