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http://hdl.handle.net/10261/162591
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dc.contributor.author | Franco-Echevarría, E. | - |
dc.contributor.author | González-Polo, Noelia | - |
dc.contributor.author | Zorrilla, Silvia | - |
dc.contributor.author | Martínez-Lumbreras, Santiago | - |
dc.contributor.author | Santiveri, Clara M. | - |
dc.contributor.author | Campos-Olivas, Ramón | - |
dc.contributor.author | Sánchez, Mar | - |
dc.contributor.author | Calvo, Olga | - |
dc.contributor.author | González, Beatriz | - |
dc.contributor.author | Pérez Cañadillas, José Manuel | - |
dc.date.accessioned | 2018-03-20T17:25:13Z | - |
dc.date.available | 2018-03-20T17:25:13Z | - |
dc.date.issued | 2017 | - |
dc.identifier | doi: 10.1093/nar/gkx685 | - |
dc.identifier | issn: 0305-1048 | - |
dc.identifier.citation | Nucleic Acids Research 45(17): 10293-10305 (2017) | - |
dc.identifier.uri | http://hdl.handle.net/10261/162591 | - |
dc.description.abstract | Transcription termination of non-coding RNAs is regulated in yeast by a complex of three RNA binding proteins: Nrd1, Nab3 and Sen1. Nrd1 is central in this process by interacting with Rbp1 of RNA polymerase II, Trf4 of TRAMP and GUAA/G terminator sequences. We lack structural data for the last of these binding events. We determined the structures of Nrd1 RNA binding domain and its complexes with three GUAA-containing RNAs, characterized RNA binding energetics and tested rationally designed mutants in vivo. The Nrd1 structure shows an RRM domain fused with a second α/β domain that we name split domain (SD), because it is formed by two non-consecutive segments at each side of the RRM. The GUAA interacts with both domains and with a pocket of water molecules, trapped between the two stacking adenines and the SD. Comprehensive binding studies demonstrate for the first time that Nrd1 has a slight preference for GUAA over GUAG and genetic and functional studies suggest that Nrd1 RNA binding domain might play further roles in non-coding RNAs transcription termination. | - |
dc.description.sponsorship | Spanish Ministry of Economy and Competitiveness (MINECO) [BFU2014-53762-P to B.G., BFU2015-71978-REDT and BFU2013-48374-P to O.C., CTQ2011-26665 and CTQ2014-52633 to J.M.P.C.]; E.F.-E. was supported by BFU2014–53762-P grant. Funding for open access charge: MINECO. | - |
dc.publisher | Oxford University Press | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-52633-P | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-53762-P | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2015-71978-REDT | - |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-48374-P | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | The structure of transcription termination factor Nrd1 reveals an original mode for GUAA recognition | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1093/nar/gkx685 | - |
dc.relation.publisherversion | https://doi.org/10.1093/nar/gkx685 | - |
dc.date.updated | 2018-03-20T17:25:13Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | http://creativecommons.org/licenses/by-nc/4.0/ | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.pmid | 28973465 | - |
local.message.claim | 2024-01-30T18:20:32.244+0100|||rp15325|||submit_approve|||dc_contributor_author|||None | * |
local.message.claim | 2024-01-30T18:21:16.154+0100|||rp15325|||submit_approve|||dc_contributor_author|||None | * |
local.message.claim | 2024-01-30T18:21:16.154+0100|||rp15325|||submit_approve|||dc_contributor_author|||None | * |
local.message.claim | 2024-01-30T18:38:07.692+0100|||rp15325|||submit_approve|||dc_contributor_author|||None | * |
local.message.claim | 2024-01-30T18:38:07.692+0100|||rp15325|||submit_approve|||dc_contributor_author|||None | * |
local.message.claim | 2024-01-30T18:38:07.692+0100|||rp15325|||submit_approve|||dc_contributor_author|||None | * |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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