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Title: | Effect of a laminin amphiphatic sequence on DPPC ordered bilayers |
Authors: | Reig Isart, Francesca; Juvé, A.; Ortiz, A.; Sospedra, Patricia; Alsina, M. Asunción CSIC | Keywords: | Laminin Peptide Fluorescence Anisotropy |
Issue Date: | 31-Aug-2005 | Publisher: | John Wiley & Sons | Citation: | Luminescence 20(4-5): 326-330 (2005) | Abstract: | A peptide sequence, stearoyl-GESIKVAVS(NH2), related to a laminin fragment, has been synthesized. Formation of aggregates was controlled by titrating a sodium anilinonaphthalene sulphonate (ANS) solution with peptide and recording fluorescence intensity increases. The results show that this system experiences a sudden increase in fluorescence at peptide concentrations around 2.5 × 10−4 mol/L. The interaction of this hydrophobic peptide with DPPC vesicles has been studied using fluorescence techniques. Its influence on the microviscosity of bilayers was determined by studying polarization/temperature dependence for ANS and diphenyl hexatriene (DPH) fluorescent probes. With both markers the presence of peptide promotes a clear increase in anisotropy values. This indicates a rigidifying effect. Leakage studies carried out with liposomes loaded with carboxyfluorescein (CF) indicate a stabilizing effect of the peptide on bilayers, in agreement with results obtained with fluorescent probes. | Description: | 5 pages, 5 fugures, 1 table.-- PMID: 16134200 [PubMed].-- Printed version published Jul-Oct 2005.-- Isuue title: "Proceedings of the XIth International Symposium on Luminescence Spectrometry - Detection Techniques in Biomedical and Environmental Analysis - (ISLS 2004). Part 2" (Beijing, China, Sep 22-24, 2004). | Publisher version (URL): | http://dx.doi.org/10.1002/bio.851 | URI: | http://hdl.handle.net/10261/16236 | DOI: | 10.1002/bio.851 | ISSN: | 1522-7235 | E-ISSN: | 1522-7243 |
Appears in Collections: | (IQAC) Artículos |
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