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Título

Synthesis of the suspected trans-11,cis-13 conjugated linoleic acid isomer in ruminant mammary tissue by FADS3-catalyzed Δ13-desaturation of vaccenic acid

AutorGarcía, C.; Duby, C.; Catheline, D.; Toral, Pablo G. ; Bernard, Laurence; Legrand, P.; Rioux, V.
Palabras clavecis-13 conjugated linoleic acid
Fatty acid desaturase 3, Stearoyl-CoA desaturase 1
trans-11
Vaccenic acid
Fecha de publicación2017
EditorAmerican Dairy Science Association
CitaciónJournal of Dairy Science 100: 783- 796 (2017)
ResumenThe octadecadienoic conjugated linoleic acid (CLA) isomer with trans-11 and cis-13 double bonds (trans11,cis-13 CLA) has been described in ruminant milk. For now, this specific CLA is suspected to derive exclusively from ruminal biohydrogenation of dietary α-linolenic acid. However, in rodents, the fatty acid desaturase 3 (FADS3) gene was recently shown to code for an enzyme able to catalyze the unexpected Δ13- desaturation of vaccenic acid, producing a Δ11,13-CLA with all the structural characteristics of the trans11,cis-13 isomer, although no commercial standard exists for complete conclusive identification. Because the FADS3 gene has already been reported in bovine animals, we hypothesized in the present study that an alternative direct FADS3-catalyzed Δ13-desaturation of vaccenic acid in mammary tissue may therefore coexist with α-linolenic acid biohydrogenation to explain the final ruminant milk trans-11,cis-13 CLA presence. Here, we first confirm that the FADS3 gene is present in ruminant mammal genomic sequence databases. Second, we demonstrate that the Δ11,13-CLA found in milk fat and the highly probable trans-11,cis-13 CLA isomer produced by rodent FADS3 possess exactly the same structural characteristics. Then, we show that bovine mammary MAC-T and BME-UV epithelial cells express both FADS3 and stearoyl-CoA desaturase 1 (SCD1) mRNA and are able to synthesize both the suspected trans-11,cis-13 CLA and cis-9,trans-11CLA (rumenic acid) isomers when incubated with vaccenic acid. Finally, the concomitant presence of the suspected trans-11,cis-13 CLA isomer with FADS3 mRNA was shown in goat mammary tissue, whereas both were conversely very low or even absent in goat liver. Therefore, this study provides several lines of evidence that, by analogy with rumenic acid, trans-11,cis-13 CLA may originate both from ruminal biohydrogenation and from direct FADS3-catalyzed Δ13-desaturation of vaccenic acid in mammary tissue.
Descripción14 páginas, 7 figuras, 1 tabla.
Versión del editorhttp://dx.doi.org/10.3168/jds.2016-11455
URIhttp://hdl.handle.net/10261/162098
DOI10.3168/jds.2016-11455
ISSN0022-0302
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