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dc.contributor.authorCasino, Patriciaes_ES
dc.contributor.authorMiguel-Romero, Lauraes_ES
dc.contributor.authorHuesa, Juanjoes_ES
dc.contributor.authorGarcía, Pabloes_ES
dc.contributor.authorGarcía-del Portillo, Franciscoes_ES
dc.contributor.authorMarina, Albertoes_ES
dc.date.accessioned2018-03-09T08:54:46Z-
dc.date.available2018-03-09T08:54:46Z-
dc.date.issued2018-01-09-
dc.identifier.citationNucleic Acids Research 46(1):456-472 (2018)es_ES
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/10261/161893-
dc.description17 páginas, 7 figuras, 1 tablaes_ES
dc.description.abstractThe RcsCDB phosphorelay system controls an extremely large regulon in Enterobacteriaceae that involves processes such as biofilm formation, flagella production, synthesis of extracellular capsules and cell division. Therefore, fine-tuning of this system is essential for virulence in pathogenic microorganisms of this group. The final master effector of the RcsCDB system is the response regulator (RR) RcsB, which activates or represses multiple genes by binding to different promoter regions. This regulatory activity of RcsB can be done alone or in combination with additional transcriptional factors in phosphorylated or dephosphorylated states. The capacity of RcsB to interact with multiple promoters and partners, either dephosphorylated or phosphorylated, suggests an extremely conformational dynamism for this RR. To shed light on the activation mechanism of RcsB and its implication on promoter recognition, we solved the crystal structure of full-length RcsB from Salmonella enterica serovar Typhimurium in the presence and absence of a phosphomimetic molecule BeF3-. These two novel structures have guided an extensive site-directed mutagenesis study at the structural and functional level that confirms RcsB conformational plasticity and dynamism. Our data allowed us to propose a β5-T switch mechanism where phosphorylation is coupled to alternative DNA binding ways and which highlights the conformational dynamism of RcsB to be so pleiotropic.es_ES
dc.description.sponsorshipSpanish Government (Ministry of Economy and Competitiveness) Grants [BIO2013–42619-P, BIO2016–78571-P to A. M, BFU2016–78606-P to P.C, BIO2016–77639-P to F.G.dP]; Valencian Government Grant Prometeo [II/2014/029 to A.M.]; Ministry of Economy and Competitiveness Ramón y Cajal contract (to P.C.); Diamond Light Source block allocation group (BAG) Proposal [MX10121]; Spanish Synchrotron Radiation Facility ALBA Proposal [2015071314]. Funding for open access charge: Spanish Government (Ministry of Economy and Competitiveness) Grants [BIO2013-42619-P, BIO2016-78571-P to A. M, BIO2016-77639-P to F.G.dP, BFU2016-78606-P to P.C.]; Valencian Government grant Prometeo [II/2014/029 to A.M.].es_ES
dc.language.isoenges_ES
dc.publisherOxford University Presses_ES
dc.relationMINECO/BIO2013/42619-Pes_ES
dc.relationMINECO/BIO2016/78571-Pes_ES
dc.relationMINECO/BFU2016/78606-Pes_ES
dc.relationMINECO/BIO2016/77639-Pes_ES
dc.relationGOVAL/Prometeo/II/2014/029es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.titleConformational dynamism for DNA interaction in the Salmonella RcsB response regulatores_ES
dc.typeartículoes_ES
dc.identifier.doi10.1093/nar/gkx1164-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1093/nar/gkx1164es_ES
dc.identifier.e-issn1362-4962-
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderGeneralitat Valencianaes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003359es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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