Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/161677
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial Guinea 15 years after its emergence

AuthorsPérez-Lago, Laura; Herranz, Marta; Comas, Iñaki CSIC ORCID ; Sierra, Olalla; Bouza, Emilio; García de Viedma, Darío
KeywordsTuberculosis
MDR
Equatorial Guinea
Screening
Emergence
Issue Date13-Oct-2016
PublisherElsevier
CitationClinical Microbiology and Infection 23(2): 92-97 (2017)
Abstract[Objective]: Molecular epidemiology techniques in tuberculosis (TB) can identify high-risk strains that are actively transmitted. We aimed to implement a novel strategy to optimize the identification and control of multidrug-resistant (MDR) TB in a specific population. [Methods]: We developed a strain-specific PCR tailored from whole genome sequencing (WGS) data to track a specific MDR prevalent strain in Equatorial Guinea (EG-MDR). [Results]: The PCR was applied prospectively on remnants of GeneXpert reaction mixtures owing to the lack of culture facilities in Equatorial Guinea. In 147 (93%) of 158 cases, we were able to differentiate between infection by the EG-MDR strain or by any other strain and found that 44% of all rifampicin-resistant TB cases were infected by EG-MDR. We also analysed 93 isolates obtained from Equatorial Guinea 15 years ago, before MDR-TB had become the problem it is today. We found that two of the scarce historical MDR cases were infected by EG-MDR. WGS revealed low variability-six single nucleotide polymorphisms acquired by this strain over 15 years-likely because of the lack in the country of a specific program to treat MDR-TB. [Conclusions]: Our novel strategy, which integrated WGS analysis and strain-specific PCRs, represents a low-cost, rapid and transferable strategy that allowed a prospective efficient survey and fast historical analysis of MDR-TB in a population.
Descriptionet al.
Publisher version (URL)http://dx.doi.org/10.1016/j.cmi.2016.10.006
URIhttp://hdl.handle.net/10261/161677
DOI10.1016/j.cmi.2016.10.006
ISSN1198-743X
E-ISSN1469-0691
Appears in Collections:(IBV) Artículos

Files in This Item:
File Description SizeFormat
2017 Clin Microbiol Infect_vers_Aut.pdf135,88 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

SCOPUSTM   
Citations

10
checked on Jan 24, 2022

WEB OF SCIENCETM
Citations

10
checked on Jan 18, 2022

Page view(s)

285
checked on Jan 24, 2022

Download(s)

184
checked on Jan 24, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.