English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/161632
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Three-dimensional analysis of synapses in the transentorhinal cortex of Alzheimer’s disease patients

AuthorsDomínguez-Álvaro, M.; Montero-Crespo, M.; Blazquez-Llorca, L.; Insausti, R.; Felipe, Javier de; Alonso-Nanclares, Lidia
Issue Date2-Mar-2018
PublisherBioMed Central
CitationActa Neuropathologica Communications 6(1): 20 (2018)
AbstractAbstract Synaptic dysfunction or loss in early stages of Alzheimer’s disease (AD) is thought to be a major structural correlate of cognitive dysfunction. Early loss of episodic memory, which occurs at the early stage of AD, is closely associated with the progressive degeneration of medial temporal lobe (MTL) structures of which the transentorhinal cortex (TEC) is the first affected area. However, no ultrastructural studies have been performed in this region in human brain samples from AD patients. In the present study, we have performed a detailed three-dimensional (3D) ultrastructural analysis using focused ion beam/scanning electron microscopy (FIB/SEM) to investigate possible synaptic alterations in the TEC of patients with AD. Surprisingly, the analysis of the density, morphological features and spatial distribution of synapses in the neuropil showed no significant differences between AD and control samples. However, light microscopy studies showed that cortical thickness of the TEC was severely reduced in AD samples, but there were no changes in the volume occupied by neuronal and glial cell bodies, blood vessels, and neuropil. Thus, the present results indicate that there is a dramatic loss of absolute number of synapses, while the morphology of synaptic junctions and synaptic spatial distribution are maintained. How these changes affect cognitive impairment in AD remains to be elucidated.
Publisher version (URL)http://dx.doi.org/10.1186/s40478-018-0520-6
Appears in Collections:(IC) Artículos
Files in This Item:
File Description SizeFormat 
40478_2018_Article_520.pdf11,98 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.