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Nucleic acids for biosensing applications

AutorAviñó, Anna; Huertas, César S.; Lechuga, Laura M. ; Eritja Casadellà, Ramón
Fecha de publicación2017
CitaciónNyNA 2017
ResumenDNA biosensors are small devices which intimately couple biological recognition element interacting with the target with a physical transducer that translates the biorecognition event into a useful electrical signal. DNA biosensors, based on nucleic acid recognition processes, are being developed towards the goal of rapid, simple and inexpensive testing of genetic or infectious diseases. In one hand, we present a biosensing approach for the label-free detection of nucleic acid sequences with special emphasis on targeting RNA sequences with secondary structures or microRNAs that are involved in several diseases. The approach is based on selecting 8-aminopurine-modified parallel-stranded DNA tail-clamps as affinity bioreceptors. These receptors have the ability of creating stable triple-stranded helices at neutral pH upon hybridization with the nucleic acid target. A surface plasmon resonance biosensor has been used for the detection. On the other hand, the design of stimuli-responsive nanoscopic gated systems involving biomolecules has recently attracted great attention. Capped materials have been mainly used in drug delivery applications. However in sensing are less common. Nucleic acids aptamers are especially attractive for the design of gated nanosensors for sensing applications. Specifically, we have prepared an aptamer-gated delivery system for the fluorogenic detection of thrombin. Similarly, tail-clamps gated system was used for the detection of miRNA.
DescripciónResumen del póster presentado al VIII International Congress on Analytical Nanoscience and Nanotechnology, celebrado en Barcelona (España) del 3 al 5 de julio de 2017.-- et al.
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