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Title

Toward integrated detection and graphene-based removal of contaminants in a lab-on-a-chip platform

AuthorsChalupniak, Andrzej ; Merkoçi, Arben
Issue Date2017
CitationNyNA 2017
AbstractIn this work a miniaturized microfluidic platform for the simultaneous detection and removal of polybrominated diphenyl ethers (PBDEs) is developed. This device consists of a polydimethylsiloxane (PDMS) microfluidic chip for an immunoreaction step, a PDMS chip with an integrated screen-printed electrode (SPCE) for detection and a PDMS-reduced graphene oxide (rGO) chip for physical adsorption and subsequent removal of PBDE residues. The detection was based on competitive immunoassay (PBDE vs. HRP-PBDE) followed by the monitoring of enzymatic oxidation of o-aminophenol (o-AP) using square wave anodic stripping voltammetry (SW-ASV). PBDE was detected with the limit of detection similar to that obtained with a commercial colorimetric test (0.018 ppb), but with the advantage of using lower reagent volumes and a reduced analysis time. The use of microfluidic chips also provides improved linearity and a better reproducibility in comparison to those obtained with batch-based measurements using screen-printed electrodes. In order to design a detection system suitable for hazardous compounds such as PBDEs, a reduced graphene oxide–PDMS composite was developed and optimized to obtain increased adsorption (based on both the hydrophobicity and π–π stacking between rGO and PBDE molecules) compared to those of non-modified PDMS. To the best of our knowledge, this is the first demonstration of electrochemical detection of flame retardants and a novel application of the rGO-PDMS composite in a biosensing system. This system can be easily adjusted to detect any analyte using the appropriate immunoassay and it supports operation in complex matrices such as seawater.
DescriptionResumen del trabajo presentado al VIII International Congress on Analytical Nanoscience and Nanotechnology, celebrado en Barcelona (España) del 3 al 5 de julio de 2017.
URIhttp://hdl.handle.net/10261/161279
Appears in Collections:(CIN2) Comunicaciones congresos
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