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Título

Characterisation of the rhodanese enzyme in Trichoderma spp

AutorMufaddal, I. Ezzi; Pascual, J. A. ; Gould, Barry J.; Lynch, J. M.
Palabras claveBioremediation
Biodegradation
Cyanide
Cyanide catabolising enzymes
Rhodanese
Trichoderma
Fusarium
Fecha de publicación8-abr-2003
EditorElsevier
CitaciónEnzyme and Microbial Technology 32(5): 629-634 (2003)
ResumenA biochemical characterisation of the rhodanese enzyme in different strains of Trichoderma was carried out in this study. The predominantly extracellular activity of this enzyme from Trichoderma was evaluated and the kinetics of the rhodanese enzyme reaction, including pH and temperature profiles, were assessed. Studies on the rhodanese enzyme from other systems have been compared with the results of this study. The kinetics of rhodanese in certain Fusarium strains, previously reported to degrade cyanides, were also analysed and compared to the enzyme from the Trichoderma. The rhodanese enzyme in all the Trichoderma strains demonstrated a broad pH optimum generally in the range from 8.5 to 11.5, along with a wide temperature optimum of 35–55 °C. The KmCN and Vmax values ranged from 7 to 16 mM and from 0.069 to 0.093 μmole ml−1 min−1 mg protein−1, respectively, between the selected strains of Trichoderma.
Descripción6 pages, 4 figures, 2 tables.
Versión del editorhttp://dx.doi.org/10.1016/S0141-0229(03)00021-8
URIhttp://hdl.handle.net/10261/16088
DOI10.1016/S0141-0229(03)00021-8
ISSN0141-0229
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