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Identification of human immunodeficiency virus type 1 (Hiv-1) based virus-like particles by multifrequency Atomic Force Microscopy

AutorGonzález-Domínguez, Irene; Gutiérrez-Granados, Sonia; Cervera, Laura; Gòdia, Francesc; Domingo, Neus
Fecha de publicación2016
EditorCSIC-ICN Centro de Investigación en Nanociencia y Nanotecnología (CIN2)
CitaciónFuerzas y Túnel (2016)
ResumenVirus-like particles (VLPs) have become a platform for vaccine production. VLPs are composed by structural viral proteins that inherently self-assemble when expressed in a host cell. They generate biological structures similar to viral antigens and since they do not contain the viral genome, they represent a highly immunogenic and safe vaccine platform. One of the main important parameters in vaccine production is the quality of the product for which it is highly required the set up of techniques that allows specific discrimination of VLPs from cellular vesicles. Multifrecuency Atomic Force Microscopy has been applied to discriminate VLPs from cellular vesicles on samples deposited in mica surfaces, by enhancing the contrast due to nanomechanical properties of the viral proteins contained in the VLPs. Moreover, this technique permits full structural characterization of the particle by accessing the properties of the virus capsid under the lipid envelope of the VLP. This enhancement of structural resolution provided by the MF AFM modes unveils new opportunities for study of the nucleic protein capsid compositional properties in VLP samples. Moreover, the characterization of HIV-1 Gag VLPs by AFM under ambient conditions allowed differentiating between the final HIV-1 Gag VLP particles and the cellular vesicular contaminationon samples deposited on mica. Conclusively, the contrast in mechanical properties between HIV-1 Gag VLPs and its major source of contamination open a new window to develop proper and efficient purification strategies for the obteining of VLP-based vaccine candidates and quality control protocols.
DescripciónResumen del póster presentado a la 10th Conferencia Fuerzas y Túnel, celebrada en Girona (España) del 5 al 7 de septiembre de 2016.
URIhttp://hdl.handle.net/10261/160847
Aparece en las colecciones: (CIN2) Comunicaciones congresos
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