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Quantification of glucosinolates in vegetable crops of Brassica rapa by near-infrared spectroscopy

AutorObregón, Sara ; Haro Bailón, Antonio de
Palabras claveGlucosinolates
Brassica
Near-infrared spectroscopy
Gluconapin
Fecha de publicaciónnov-2016
CitaciónThe Food Factor I Barcelona Conference (2016)
ResumenIn Spain, Brassica rapa subsp. rapa L. includes turnip greens and turnip top as vegetable products. Turnip greens are the young leaves harvested in the vegetative period, while turnip tops are the fructiferous stems with the flower buds and the surrounding leaves. Both are boiled and generally consumed as meat companions. They are characterized by a particular bitter and pungent taste, which differentiate them from other vegetables in the genus Brassica, such as cabbage and cauliflower. The bitterness has been related to the content of some glucosinolate degradation products. Glucosinolates and their breakdown products are known to have important biological activity and chemoprotective effects against cancer1,2,3. The determination of glucosinolate content by the standard methods (HPLC) is expensive and time-consuming. In contrast, Near-infrared spectroscopy (NIRS) has many advantages, since analysis can be carried out with a considerable saving of time, at a low cost and without using hazardous chemicals. The purpose of this work was to test the potential of NIRS for predicting the total glucosinolate (t-GSL) content, as well as those major glucosinolates found in the leaves and stems of B. rapa. NIRS calibrations resulted in coefficients of determination and standard of prediction in external validation of 0.96 and 3.28 for t-GSL; 0.94 and 3.49 for Gluconapine; 0.41 and 1.08 for Progoitrine, and 0.55 and 0.60 for Glucobrassicanapine respectively. It was concluded that NIRS shows a high potential as an analytical method for total and individual glucosinolate routine analysis in turnip greens and turnip tops.
DescripciónTrabajo presentado en la The Food Factor I Barcelona Conference, celebrada en Barcelona 2 al 4 de noviembre de 2016.
URIhttp://hdl.handle.net/10261/160797
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