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Lipin-2 role in the differentiation and polarized activation of macrophages

AuthorsPablo, Nagore de; Balsinde, Jesús ; Balboa, María A.
Issue Date2016
CitationCIBERDEM Annual Meeting (2016)
AbstractObesity is a state of chronic low degree inflammation governed by inflammatory cells, such as macrophages, that can lead to the development of insulin resistance and hence to the establishment of the type 2 diabetes. The elevated saturated free fatty acids present in the adipose tissue of obese individuals have been described to change the activation state of macrophages from the antiinflammatory M2 polarization state, to the proinflammatory M1 polarization state. Polarized M1 macrophages in the obese adipose tissue contribute to maintain the inflammation state and the development of insulin resistance. We have recently described that Lipin-2, an enzyme that dephosphorylates phosphatidic acid to form diacylglycerol to be used for triacylglycerol synthesis, reduces the proinflammatory signaling mediated by elevated concentration of saturated fatty acids in macrophages. Based on its protective function we decided to extend our investigations to uncover whether lipin-2 could also be involved in macrophage differentiation and polarization. To that end we established an in vitro differentiation and polarization protocol for the human THP-1 promonocytic cell line to study by qPCR the expression of well-known differentiation and polarization markers in cells with different expression levels of lipin-2. Also, analysis of the expression of Lipin-2 revealed increased mRNA levels for the enzyme during macrophage differentiation and M1 polarization processes. Overall our results suggest that Lipin-2 may play an important role during differentiation/ polarization processes.
DescriptionResumen del póster presentado presentado al CIBERDEM Annual Meeting, celebrado en Cerdanyola del Vallès, Barcelona (España) del 11 al 13 de mayo de 2016.
Appears in Collections:(IBGM) Comunicaciones congresos
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