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dc.contributor.authorMartínez-Moreno, Mónicaes_ES
dc.contributor.authorMartínez-Ruiz, Antonioes_ES
dc.contributor.authorÁlvarez Barrientos, Albertoes_ES
dc.contributor.authorGavilanes, Franciscoes_ES
dc.contributor.authorLamas Peláez, Santiagoes_ES
dc.contributor.authorRodríguez-Crespo, Ignacioes_ES
dc.identifier.citationJ Biol Chem. 282(32):23044-54 (2007)es_ES
dc.description12 p.-7 fig.es_ES
dc.description.abstractCertain patients suffering from chronic diseases such as AIDS or cancer experience a constant cellular secretion of tumor necrosis factor α and other pro-inflammatory cytokines that results in a continuous release of nitric oxide (·NO) to the bloodstream. One immediate consequence of the deleterious action of ·NO is weight loss and the progressive destruction of muscular mass in a process known as cachexia. We have previously reported that caveolin-3, a specific marker of muscle cells, becomes down-regulated by the action of ·NO on muscular myotubes. We describe herein that the changes observed in caveolin-3 levels are due to the alteration of the DNA binding activity of the muscular transcription factor myogenin. In the presence of ·NO, the binding of transcription factors from cell nuclear extracts of muscular tissues to the E boxes present in the caveolin-3 promoter become substantially reduced. When we purified recombinant myogenin and treated it with ·NO donors, we could detect its S-nitrosylation by three independent methods, suggesting that very likely one of the cysteine residues of the molecule is being modified. Given the role of myogenin as a regulatory protein that determines the level of multiple muscle genes expressed during late myogenesis, our results might represent a novel mode of regulation of muscle development under conditions of nitric oxide-mediated toxicity.es_ES
dc.description.sponsorshipThis work was supported by Dirección General de Investigación Científica y Técnica Grants BFU 2006-13033, BFU 2006-05395, CP03-0025, and SAF2006-02410.es_ES
dc.publisherAmerican Society for Biochemistry and Molecular Biologyes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.titleNitric oxide down-regulates caveolin-3 levels through the interaction with myogenin, its transcription factores_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderDirección General de Investigación Científica y Técnica, DGICT (España)es_ES
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