English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/160469
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Genomic analysis of two Pseudomonas spp. strains displaying endophytic lifestyle in olive roots and effective biological control of Verticillium dahliae

AuthorsMartínez-García, Pedro Manuel; Ruano Rosa, David ; Schilirò, Elisabetta ; Gómez del Campo, María; Ramos, Cayo; Rodríguez-Palenzuela, Pablo; Mercado-Blanco, Jesús
Endophytic bacteria
Verticillium wilt
Detoxification systems
Issue DateMay-2015
Citation7th IOBC/WPRS Symposium Integrated Protection of Olive Crops (2015)
AbstractPseudomonas jluorescens PICF7 and Pseudomonas pulida PICP2 are two effective biocontrol strains against Verticillium wilt (Verticillium dahliae Kleb.) of olive. In addition, both bacteria can colonize endophytically olive roots, even simultaneously. However, our knowledge on bacteria! traits involved in biological control and endophytic lifestyle is scarce. In this study, we have sequenced and annotated the complete genomes of strains PICF7 (1 contig, 6.14 Mbp) and PICP2 (63 contigs, 5.60 Mbp). A comparative bioinformatics analysis was catTied out aiming to identify genes potentially involved in (i) biological control, (ii) plant-bacteria interaction, and (iii) endophytism. The analysis has revealed that, among other determinants frequently associated to biocontrol andlor plant-bacteria interaction, the genome of strain PICF7 harbours the genetic information to encode two type VI (T6SS) and one Type III (T3SS) secretion systems, the T3SS effectors avrE 1 and hopB 1, the siderophores pyoverdine, pyochelin and the hemophore HasAp, diverse adhesion factors, a battery of celldegrading enzymes (protease, cellulase and lipase), as well as detoxifying systems such as CopA, KatB, Dps and Cbb. On the contrary, the genome of strain PICP2 showed, among other differences, no coding region for T3SS. Sorne ofthe phenotypes predicted by genome analysis have been demonstrated by biochemical and microbiological analyses. The availability of both genomes will help to understand both biocontrol activity and the ability to colonize and persist within olive root tissues.
DescriptionTrabajo presentado en el 7th IOBC/WPRS Symposium Integrated Protection of Olive Crops, celebrado en Kalamata (Grecia) del 11 al 14 de mayo de 2015.
Appears in Collections:(IAS) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.