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dc.contributor.authorMarquina, Claraes_ES
dc.contributor.authorCorredor, Eduardoes_ES
dc.contributor.authorFuente, Jesús M. de laes_ES
dc.contributor.authorRubiales, Diegoes_ES
dc.contributor.authorTestillano, P.S.es_ES
dc.identifier.citation20th International Conference of Magnetism (2015)es_ES
dc.descriptionTrabajo presentado en la 20th International Conference of Magnetism, celebrada en Barcelona del 5 al 10 de julio de 2015.-- Marquina, Clara et al.es_ES
dc.description.abstractMagnetic nanoparticles have been implemented in biotechnological and biomedical applications related to animal (in particular human) cells and pathologies. However, their use in plant research biology is far less studied, although it could lead to the development of interesting plant biotechnology applications. For this purpose the first stage is to work out the penetration and transport of the magnetic nanoparticles into living plants and plant cells and how they interact with plant cells and tissues [1]. We have synthesized biocompatible suspensions of iron-­‐oxide carbon-­‐coated nanoparticles, obtained by a gas-­‐phase condensation method. They have been injected into pumpkin (Cucurbita pepo) living plants. The graphitic shell of our nanoparticles made possible their visualization into plant cells and tissues, using different microscopy techniques (fluorescence, confocal, light and electron microscopy). Moreover, their magnetic character allowed the nanoparticles to be positioned in the desired plant tissue by applying magnetic field gradients. In the absence of magnetic fields, the nanoparticles can as well travel along the vascular system, reaching different cell and tissues. Nanoparticles have been found both in the cytoplasm and in the extracellular space between cells. A size-­‐based selection mechanism seems to be operating, probably involving cell walls and waxes acting as a barrier. Accumulation of nanoparticles was detected in leaf trichomes, suggesting a way for nanoparticles excretion/detoxification. With respect to cytotoxicity, it has been observed that cells containing nanoparticle agglomerates exhibited a cytoplasm denser than that of cells containing few nanoparticles. Damage at the plant level was not macroscopically evident. Although more studies are necessary to unveil the nanoparticles penetration and translocation mechanisms as well as their cyto-­‐ and phyto-­‐toxicity, our results open a wide range of possibilities for using magnetic nanoparticles in general plant research and agronomy.es_ES
dc.title"In -planta" penetration and translocation of Fe@C magnetic nanoparticleses_ES
dc.typeComunicación de congresoes_ES
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