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De novo genome and transcriptome sequencing combined with differential expression analysis identify putative pathogenicity factors from Ascochyta rabiei

AuthorsFondevilla, Sara ; Krezdorn, Nicolas; Huettel, B.; Hoffmeier, K.; Terauchi, R.; Rotter, Björn; Winter, Peter; Kahl, Guenter
Issue DateOct-2016
CitationIV International Ascochyta Workshop (2016)
AbstractEfforts to control ascochyta blight in chickpea have mostly been based on the identification of resistance genes in the host. The identification of A. rabiei pathogenicity determinants will open a wide range of new alternatives to control ascochyta blight disease and increase our knowledge about Ascochyta-legume interactions. However, very little is known about the pathogenicity factor of A. rabiei. Equally, little information about A. rabiei is available at the molecular level. We have used Illumina and PacBio RS II platforms for sequencing the genome and transcriptome of an isolate of A. rabiei belonging to pathotype IV. A. rabiei secretome was also predicted. In addition, an accurate transc riptomic analysis using Massive Analysis of cDNA Ends (MACE) was performed to compare the gene expression profile of the fungus infecting chickpea with that of the fungus growing in absence of its host (1). A detailed analysis of the genes up-regulated dur ing infection identified a collection of candidate pathogenicity factors and unravelled the strategies that allow the pathogen to cause disease: A. rabiei produces a battery of cell wall-degrading enzymes, used by the pathogen to penetrate its host, secret es toxins to kill the host cells, and is able to detoxify the fungitoxic compounds produced by the plant as defense.
DescriptionTrabajo presentado en el IV International Ascochyta Workshop (Ascochyta 2016), celebrado en Tróia (Portugal) el 10 y 11 de octubre de 2016.
Appears in Collections:(IAS) Comunicaciones congresos
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