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http://hdl.handle.net/10261/160254
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Título: | Selective T cell subset depletion with anti-CD4 and anti-CD8 intact ricin immunotoxins |
Autor: | Izquierdo, Manuel CSIC ORCID ; Balboa, María A. CSIC ORCID; Figuera, Angela; López-Botet, Miguel | Palabras clave: | Immunotoxins T cells Bone marrow purging |
Fecha de publicación: | 1988 | Editor: | John Wiley & Sons British Society for Immunology |
Citación: | Clinical and Experimental Immunology 74(2): 300-304 (1988) | Resumen: | In the present study we have comparatively analysed the specific activity of a panel of immunotoxins (ITs) prepared by coupling Ricin to several monoclonal antibodies (MoAbs) directed against the CD3, CD4 and CD8 T cell membrane molecules. Peripheral blood and bone marrow mononuclear cells (PBMC and BMMC) were incubated with the different ITs for 2 h, in the presence of 0.1 M lactose, washed and subsequently stimulated with either phytohemagglutinin (PHA) or an anti-CD3 MoAb. Our results indicate that the proliferative response of PBMC to both stimuli was specifically inhibited (>95%) by either anti-CD3 IT or a combination of anti-CD4 and CD8 ITs, at concentrations comparable to those previously used for ex vivo T cell depletion (300 ng/ml). When used individually at the same dose, anti-CD8 and anti-CD4 ITs inhibited the PHA-induced PBMC proliferative response 40 and 70% respectively. When either anti-CD4 or anti-CD8 IT-treated cells were activated with PHA and cultured for 14 days in the presence of IL-2, less than 2% of the blasts expressed the corresponding antigens as assessed by flow cytometry analysis. Similar results were observed when BMMC were treated with the different ITs. In contrast, the growth of CFU-GM was minimally affected (0-25% inhibition). Our data indicate that ITs directed against T cell subsets are highly active and specific reagents that may be potentially useful for pre-transplant bone marrow purging. | URI: | http://hdl.handle.net/10261/160254 | Identificadores: | issn: 0009-9104 e-issn: 1365-2249 |
Aparece en las colecciones: | (IIBM) Artículos (IBGM) Artículos |
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