English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/160217
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Microencapsulation with alginate/CaCO 3: A strategy for improved phage therapy

AuthorsColom, Joan; Cano-Sarabia, Mary ; Otero, Jennifer; Aríñez-Soriano, Javier; Cortés, Pilar; Maspoch, Daniel ; Llagostera, Montserrat
Issue Date2017
PublisherNature Publishing Group
CitationScientific Reports 7: 41441 (2017)
AbstractBacteriophages are promising therapeutic agents that can be applied to different stages of the commercial food chain. In this sense, bacteriophages can be orally administered to farm animals to protect them against intestinal pathogens. However, the low pH of the stomach, the activities of bile and intestinal tract enzymes limit the efficacy of the phages. This study demonstrates the utility of an alginate/CaCO encapsulation method suitable for bacteriophages with different morphologies and to yield encapsulation efficacies of ∼100%. For the first time, a cocktail of three alginate/CaCO-encapsulated bacteriophages was administered as oral therapy to commercial broilers infected with Salmonella under farm-like conditions. Encapsulation protects the bacteriophages against their destruction by the gastric juice. Phage release from capsules incubated in simulated intestinal fluid was also demonstrated, whereas encapsulation ensured sufficient intestinal retention of the phages. Moreover, the small size of the capsules (125-150 μm) enables their use in oral therapy and other applications in phage therapy. This study evidenced that a cocktail of the three alginate/CaCO-encapsulated bacteriophages had a greater and more durable efficacy than a cocktail of the corresponding non-encapsulated phages in as therapy in broilers against Salmonella, one of the most common foodborne pathogen.
Publisher version (URL)https://doi.org/10.1038/srep41441
URIhttp://hdl.handle.net/10261/160217
DOI10.1038/srep41441
Identifiersdoi: 10.1038/srep41441
e-issn: 2045-2322
Appears in Collections:(CIN2) Artículos
Files in This Item:
File Description SizeFormat 
Microencap.pdf1 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.