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Candidate gene expression analysis of the genes located in the QTLAR3 in chickpea related with Ascochyta blight resistance

AuthorsMadrid, Eva CSIC ORCID; Gil, Juan; Barro Losada, Francisco CSIC ORCID ; Millán, Teresa; Rubio, Josefa
Issue DateJul-2014
Citation6th International Food Legume Research Conference (2014)
7th International Conference of Legume Genetics and Genomics (2014)
AbstractRecently, the location of the QTLAR3, related with resistance to pathotype I of Ascochyta rabiei in chickpea, has been redefined using an interespecific recombinant inbred line population (ILC72xCr5-10). The location of the QTLAR3 peak was linked to an ethylene insensitive 3-like gene (Ein3), explaining 44.3% of the total phenotypic variation for resistance to blight. This genomic region was predicted to be at the Ca2 physical position 32-33 Mb, comprising 42 genes. Candidate genes located in this region include Ein3, Avr9/Cf9 and Argonaute 4, directly involved in disease resistance mechanisms (Madrid et al. 2014). Two parental lines (ILC72 and Cr5-10) were incoculated with PathI and total RNA was extracted in both inoculated and non-inoculated leaves. In an attempt to elucidate the implication of these three genes in blight resistance, the temporal expression patterns at 12, 24 and 48 hour after inoculation (h.a.i.) were investigated doing qPCR. Preliminary analysis revealed transcriptional changes in response to Ascochyta blight for the three genes. Differential transcript accumulation was observed for all the genes in respect with the susceptible genotype. An induction of the expression was observed in ILC72 for both Ein3 and Argonaute 4 at 24 h.a.i whereas the susceptible genotype (Cr5-10) did not show differential expression at the different timepoints analyzed. Avr9/Cf9 showed activation at 12 h.a.i. reaching basal levels at 24 h.a.i. in the resistant genotype.
DescriptionTrabajo presentado en la 6th International Food Legume Research Conference y la 7th International Conference of Legume Genetics and Genomics (IFLRC VI & ICLGG VII), celebrada en Saskatoon (Canadá) del 7 al 11 de julio de 2014.
Appears in Collections:(IAS) Comunicaciones congresos

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