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Large-scale transcriptome analysis in faba bean (Vicia faba L.)

AuthorsOcaña-Moral, Sara; Seoane, Pedro; Bautista, Rocío; Torres, Ana M.; Claros, Gonzalo M.; Madrid, Eva
Issue DateJul-2014
Citation6th International Food Legume Research Conference (2014)
7th International Conference of Legume Genetics and Genomics (2014)
AbstractAlthough faba bean is one of the biggest traded pulse crops, genomic resources are still limited. A comprehensive characterization of the faba bean transcriptome was performed using the Illumina platform. Two faba bean genotypes (Vf29H and Vf136) with differential reaction to Ascochyta fabae and 106.397 ESTs found in different public databases were used for de novo transcriptome assembly using a work flow with OASES, and CAP3. The resulting unigenes were annotated with Full-LengtherNext. The preliminary analysis of the 120.922 unigenes collection revealed that 20,804 out of the 39,269 were non-redundant transcripts based on orthologue ID. 2,752putative ncRNAs were found. Futhermore, 7,165 unigenes lacking homology could be considered as putative legume-specific genes. Single nucleotide polymorphisms identified through data mining provide a database for genotyping and integration of translational genomics in breeding programmes. In addition, the faba bean and chickpea transcriptome alignment revealed that 81.27% of the faba bean transcripts where present in the chickpea transcriptome despite of using a single faba bean source of tissue. As the genotypes sequenced show differential reaction to A. fabae and the cDNA libraries were constructed using inoculated and non-inoculated leaves, genes with differential expression in both genotypes were characterized. Thus, 348 and 327 transcripts where found to be unique in resistant and susceptible genotypes, respectively. On the other hand, 212 and 282 transcripts were found to be overexpressed in both lines. These transcripts might represent candidate genes associated with the A. fabae resistance.
DescriptionTrabajo presentado en la 6th International Food Legume Research Conference y la 7th International Conference of Legume Genetics and Genomics (IFLRC VI & ICLGG VII), celebrada en Saskatoon (Canadá) del 7 al 11 de julio de 2014.
Appears in Collections:(IAS) Comunicaciones congresos
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