English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/160136
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Decrease in pH destabilizes individual vault nanocages by weakening the inter-protein lateral interaction

AuthorsLlauró, Aida; Guerra, Pablo ; Kant, Ravi; Bothner, Brian; Verdaguer, Núria ; Pablo, Pedro J. de
Issue Date14-Oct-2016
PublisherNature Publishing Group
CitationScientific Reports 6: 34143 (2016)
AbstractVault particles are naturally occurring proteinaceous cages with promising application as molecular containers. The use of vaults as functional transporters requires a profound understanding of their structural stability to guarantee the protection and controlled payload delivery. Previous results performed with bulk techniques or at non-physiological conditions have suggested pH as a parameter to control vault dynamics. Here we use Atomic Force Microscopy (AFM) to monitor the structural evolution of individual vault particles while changing the pH in real time. Our experiments show that decreasing the pH of the solution destabilize the barrel region, the central part of vault particles, and leads to the aggregation of the cages. Additional analyses using Quartz-Crystal Microbalance (QCM) and Differential Scanning Fluorimetry (DSF) are consistent with our single molecule AFM experiments. The observed topographical defects suggest that low pH weakens the bonds between adjacent proteins. We hypothesize that the observed effects are related to the strong polar character of the protein-protein lateral interactions. Overall, our study unveils the mechanism for the influence of a biologically relevant range of pHs on the stability and dynamics of vault particles.
Publisher version (URL)https://www.doi.org/10.1038/srep34143
Identifiersdoi: 10.1038/srep34143
issn: 2045-2322
Appears in Collections:(IBMB) Artículos
Files in This Item:
File Description SizeFormat 
Decrease_pH_desestabilizes-Llauro.pdf1,35 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.