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Título

Centrosome Amplification Increases Single-Cell Branching in Post-mitotic Cells

AutorRicolo, Delia; Deligiannaki, Myrto; Casanova, Jordi ; Araújo, Sofia J.
Palabras claveSas-4
Emi1
Rca1
Centriole
Centrosome
Lumen
Subcellular
Trachea
Drosophila
Branching
Fecha de publicación29-sep-2016
EditorElsevier
CitaciónCurrent Biology 26(20): 2805-2813 (2016)
ResumenCentrosome amplification is a hallmark of cancer, although we are still far from understanding how this process affects tumorigenesis [1, 2]. Besides the contribution of supernumerary centrosomes to mitotic defects, their biological effects in the post-mitotic cell are not well known. Here, we exploit the effects of centrosome amplification in post-mitotic cells during single-cell branching. We show that Drosophila tracheal cells with extra centrosomes branch more than wild-type cells. We found that mutations in Rca1 and CycA affect subcellular branching, causing tracheal tip cells to form more than one subcellular lumen. We show that Rca1 and CycA post-mitotic cells have supernumerary centrosomes and that other mutant conditions that increase centrosome number also show excess of subcellular lumen branching. Furthermore, we show that de novo lumen formation is impaired in mutant embryos with fewer centrioles. The data presented here define a requirement for the centrosome as a microtubule-organizing center (MTOC) for the initiation of subcellular lumen formation. We propose that centrosomes are necessary to drive subcellular lumen formation. In addition, centrosome amplification increases single-cell branching, a process parallel to capillary sprouting in blood vessels [3]. These results shed new light on how centrosomes can contribute to pathology independently of mitotic defects.
URIhttp://hdl.handle.net/10261/160104
Identificadoresdoi: 10.1016/j.cub.2016.08.020
issn: 0960-9822
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