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Bacillus onubensis sp. nov., isolated from the air of two Andalusian caves

AutorDomínguez-Moñino, Irene ; Jurado, Valme ; González Pimentel, José L.; Miller, A. Z. ; Hermosín, Bernardo ; Sáiz-Jiménez, Cesáreo
Palabras claveBacillus
Bacillus onubensis
Show cave
16S rRNA gene sequence
Polyphasic taxonomy
Fecha de publicación31-ene-2018
EditorElsevier
CitaciónSystematic and Applied Microbiology (2018) Article in Press
ResumenTwo Gram-positive, catalase-positive, oxidase-negative, motile, endospore-forming, rod-shaped bacteria, designated as 0911MAR22V3T and 0911TES10J4, were isolated from air samples collected in two show caves, located in Andalusia, Southern Spain. Phylogenetic analysis based on 16S rRNA gene sequences indicated that both strains were indistinguishable and they were most closely related to Bacillus humi DSM 16318T (98%). DNA-DNA hybridization values of the strain 0911MAR22V3T with respect to strain 0911TES10J4 and B. humi DSM 16318T were 76.8% (73.9%, reciprocal) and 56.9% (63.3%, reciprocal analysis), respectively. Whole genome average nucleotide identity (ANI) values of both strains were in the threshold value for species delineation and less than 85% with B. humi. Strains 0911MAR22V3T and 0911TES10J4 grew at 10-47. °C (optimum 37. °C), at pH 6-9.5 and with 0-8% (w/v) NaCl (optimum 1%). In both strains the dominant isoprenoid quinone was MK-7, the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and two more phospholipids, the predominant fatty acids were iso-C15:0 and anteiso-C15:0 and the DNA G + C content was 38. mol%. On the basis of their phylogenetic relatedness and their phenotypic and genotypic features, the strains 0911MAR22V3T and 0911TES10J4 should be attributed to a novel species within the genus Bacillus, for which the name Bacillus onubensis sp. nov. is proposed. The type strain is 0911MAR22V3T (=LMG 27963T = CECT 8479T); and strain 0911TES10J4 (CECT 8478) is a reference strain.
Descripción19 páginas.-- 2 figuras.-- 2 tablas.-- 43 referencias.-- Article in Press.-- Irene Dominguez-Moñino, Valme Jurado, Jose L.Gonzalez-Pimentel, Ana Zelia Miller, Bernardo Hermosin, Cesareo Saiz-Jimenez, Bacillus onubensis sp.nov., isolated from the air of two Andalusian caves, Systematic and Applied Microbiology https://doi.org/10.1016/j.syapm.2018.01.001
Versión del editorhttps://doi.org/10.1016/j.syapm.2018.01.001
URIhttp://hdl.handle.net/10261/160092
DOI10.1016/j.syapm.2018.01.001
ISSN0723-2020
E-ISSN1618-0984
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