English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/159868
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Quantitative assessment of natural toxicity in sponges: toxicity bioassay versus compound quantification

AutorMartí, Ruth; Fontana, A.; Uriz, María Jesús ; Cimino, C.
Palabras claveDysidea avara
Ircinia variabilis
Natural toxicity
Sesterpene
Avarol
Palinurin
Secondary metabolites
Crude extracts
Bioactivity
Bioassays
Microtox
Chemical quantification
Sponges
Fecha de publicación2003
EditorSpringer
CitaciónJournal of Chemical Ecology 29(6):1307-1318 (2003)
ResumenMicrotox assay was used to assess the natural toxicity of two sponges, Dysidea avara and Ircinia variabilis. The activity of crude extracts and major metabolites were compared. Methanol extract of D. avara was more toxic than that of acetone and was as toxic as pure avarol, thus suggesting that the toxicity of the sponge was mainly due to this metabolite. We also quantified palinurin, the major metabolite of I. variabilis, in specimens from several habitats. With the same methanol extracts used for palinurin quantification, we ran the Microtox assay and found a positive significant regression between toxicity and concentration of this metabolite. Pure palinurin was tested at the same concentration present in the extract, and the toxicity recorded was higher than that of the methanol extract. As with avarol from D. avara, palinurin is the main secondary metabolite that confers toxicity to I. variabilis. The results confirm that the standardized Microtox assay is an accurate and reproducible tool for assessing the toxicity of crude extracts and pure metabolites of marine organisms. These results also suggest that methanol is more suitable than acetone for the detection of species toxicity by Microtox. The method is faster and easier to perform than chemical quantification even when the sponge chemistry is known, and is appropriate for studies on variation in natural toxicity over a range of environmental conditions.
DescripciónEste artículo contiene 12 páginas, 4 figuras,1 tabla.
Versión del editorhttps://link.springer.com/article/10.1023/A:1024201100811
URIhttp://hdl.handle.net/10261/159868
ISSN0098-0331
E-ISSN1573-1561
Aparece en las colecciones: (CEAB) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.