English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/159824
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Diagnosis of the infection of sunflower by Orobanche cumana using multicolour fluorescence imaging

AutorPérez-Bueno, María Luisa; Barón Ayala, Matilde; García-Carneros, Ana B. ; Molinero-Ruiz, Leire
Palabras claveEarly detection
Helianthus annuus L.
Multicolour fluorescence
Secondary metabolites
Fecha de publicación2014
EditorInternational Sunflower Association
Asociación Española del Girasol
CitaciónProceedings of the Third International Symposium on Broomrape ( Orobanche spp.) in Sunflower: 100-103 (2014)
ResumenOrobanche cumana is an holoparasite and thus totally dependent on sunflower for fixed carbon. Initial stages of the infection occur in the first weeks after sowing and are critical for the establishment of a continuum between the host and the parasite vascular system. From that moment the parasite obtains its supply of water, mineral nutrients and assimilates from the host plant. Alterations of plant photosynthesis can be detected using remote sensing techniques for detection of fluorescence emitted by plants. One of these indirect techniques is multicolour fluorescence imaging. In this work we assessed the early infection of sunflower by O. cumana using multicolour fluorescence imaging and we inferred physiological processes affected in sunflower plants infected by the parasite. Ten germinated seeds of the inbred line NR5 were inoculated with population LP2013 of O. cumana. The same number of not inoculated seeds was used as control. Sunflower was planted in pots with soil mixture and grown in greenhouse at 12-22ºC for six weeks. Multicolour fluorescence imaging was conducted three, four and five weeks after inoculation. The two first pairs of fully expanded leaves of each sunflower plant were imaged, and, for each measure date, five fluorescence variables in inoculated plants were compared to those in the control. Three weeks after inoculation, when symptoms of infection were still not observed, decreased levels of blue and green fluorescence and increased far-red fluorescence were observed in leaves of the inoculated plants. In subsequent measures four and five weeks after inoculation, when inoculated plants displayed symptoms of infection by O. cumana, differences of fluorescence between inoculated plants and the controls were the same and statistically supported. Our results show an increase in total chlorophyll content of sunflower plants infected by O. cumana which is probably related to the need of higher photosynthetic activity in order to supply the parasite with photosynthate. Biochemical mechanisms underlying photosynthesis impairment must be further investigated. The results obtained three weeks after inoculation show that multicolour fluorescence imaging can detect fluorescence differences in inoculated sunflower at early time. Therefore, this technique can be used as a diagnostic tool for early detection of genotypes of sunflower which are susceptible or resistant to O. cumana.
DescripciónTrabajo presentado en el Third Internacional Symposium on broomrape (Orobanche spp.) in Sunflower, celebrado en Córdoba (España) del 3 al 6 de junio de 2014.
Aparece en las colecciones: (EEZ) Libros y partes de libros
(IAS) Libros y partes de libros
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.