English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/159476
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorPuertas, A. I.es_ES
dc.contributor.authorIbarburu, Idoiaes_ES
dc.contributor.authorElizaquível, P.es_ES
dc.contributor.authorZuriarrain, Andonies_ES
dc.contributor.authorBerregi, I.es_ES
dc.contributor.authorLópez, Palomaes_ES
dc.contributor.authorPrieto, Aliciaes_ES
dc.contributor.authorAznar, Rosaes_ES
dc.contributor.authorDueñas, María Teresaes_ES
dc.date.accessioned2018-01-23T11:58:22Z-
dc.date.available2018-01-23T11:58:22Z-
dc.date.issued2018-04-
dc.identifier.citationLWT 90:469-474 (2018)es_ES
dc.identifier.issn0023-6438-
dc.identifier.urihttp://hdl.handle.net/10261/159476-
dc.description24 p.-2 fig.-2 tab.-1 fig. supl.-1 tab.supl.es_ES
dc.description.abstractLactic acid bacteria (LAB) are naturally occurring bacterial populations in traditional cider from the Basque Country (Spain), which is spontaneously fermented without starters. Some LAB are able to produce exopolysaccharides (EPS) thus causing viscosity, a cider spoilage called “ropiness” or “oilness”. A total of 41 isolates recovered between 1992 and 2009 from ropy ciders of different cider factories were included in the present study. Identification by 16S rRNA gene sequencing, biochemical traits (API), RAPD-PCR using four universal primers (M13, M13V, P1 and P2) and 16S-23S ISRs amplification profiles revealed that the spoiler species were L. collinoides, L. diolivorans, L. sicerae and L. suebicus. EPS production in L. collinoides and L. sicerae strains approached by PCR amplification of eps genes encoding for homopolysaccharide and heteropolysaccharide revealed that these strains synthesize heteropolysacccharides and in addition, L. collinoides CUPV231 synthesizes a 2-branched (1,3)-β-D-glucan. The molecular weight of their EPS determined by HP-SEC revealed the presence of three polymers with average molecular weights (MW) of around 106 g/mol, 104 g/mol and 103 g/mol. The middle-weight fraction was the most abundant except in L. sicerae CUPV261 which mainly produced the high Mw polymer.es_ES
dc.description.sponsorshipThis study was supported by grants AGL2012-40084-C03 (co-funded by FEDER), AGL2015-65010-C3-1-R, IT866-13 from the Basque Government and PROMETEO/2012/040 from the Generalitat Valenciana.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccessen_EN
dc.subjectCideres_ES
dc.subjectLactobacilluses_ES
dc.subjectExopolysaccharides (EPS)es_ES
dc.subjectRopy isolateses_ES
dc.titleDisclosing diversity of exopolysaccharide-producing lactobacilli from Spanish natural ciderses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.lwt.2017.12.048-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.lwt.2017.12.048es_ES
dc.embargo.terms2019-04es_ES
dc.rights.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderEusko Jaurlaritzaes_ES
dc.contributor.funderGeneralitat Valencianaes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003359es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003086es_ES
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Puertas et al LWT aceptado 2018.pdfPostprint1,1 MBAdobe PDFThumbnail
View/Open
Show simple item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.